p53 is a rate-limiting factor in the repair of higher-order DNA structure

被引:19
作者
Aranda-Anzaldo, A
Orozco-Velasco, F
Garcia-Villa, E
Gariglio, P
机构
[1] Inst Politecn Nacl, CINVESTAV, Dept Genet & Biol Mol, Mexico City 07000, DF, Mexico
[2] UAEM, Fac Med, Mol Biol Lab, Mexico City 50000, DF, Mexico
[3] IPN, CICATA, Mexico City 11500, DF, Mexico
来源
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1999年 / 1446卷 / 03期
关键词
DNA loop; DNA repair; DNA topology; nuclear matrix; p53;
D O I
10.1016/S0167-4781(99)00086-X
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The product of the p53 tumor suppressor gene has been implicated in safeguarding genomic stability by transactivating genes involved in cell cycle arrest, repair of DNA damage or induction of apoptosis. Several properties of p53 suggest that it might be directly involved in DNA repair processes. Eukaryotic DNA is highly organized in supercoiled loops anchored to the nuclear matrix. This organization is very important for cell function and survival, suggesting that repair of DNA damage must include both, the integrity of the double helix and the complex DNA topology. In this work, we studied the kinetics and efficiency of higher-order DNA structure repair in cells with normal and reduced levels of p53, and present evidence suggesting that p53 may be involved in the stabilization and/or repair of higher-order DNA structure. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:181 / 192
页数:12
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