Synthetic nucleoside and nucleotides .34. Photoaffinity labeling of HIV reverse transcriptase: Synthesis and utilization of 2',3'-dideoxy uridylate analogs bearing aryl(trifluoromethyl)-diazirine moiety

被引：9

作者：

Yamaguchi, T

Saneyoshi, M

机构：

[1] Department of Biological Sciences, Nishi-Tokyo University, Kitatsuru-gun, Yamanashi 409-01, 2525 Yatsuzawa, Uenohara-machi

来源：

NUCLEOSIDES & NUCLEOTIDES | 1996年 / 15卷 / 1-3期

关键词：

D O I：

10.1080/07328319608002409

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In order to develop a photoaffinity labeling reagent for HIV-1 reverse transcriptase, a 2',3'-dideoxyUTP analog bearing a photo-reactive group at the 5-position of the uracil ring, 2',3'-dideoxy-E-5-[4-[3-(trifluoromethyl)-3H-diazirin-3-yl]styryl]-UTP, was designed and synthesized. This photo-reactive analog could preferentially bind to the 66 kDa subunit of the p66/p51 heterodimeric HIV-1 reverse transcriptase under irradiation by near-ultraviolet light (365 nm).

引用

页码：607 / 618

页数：12

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