Use of capillary blood to diagnose hereditary spherocytosis

被引：13

作者：

Crisp, Renee L. ^{[1
,2
,3
]}

Solari, Liliana ^{[4
]}

Gammella, Daniel ^{[4
]}

Schvartzman, Gabriel A. ^{[3
]}

Cristina Rapetti, Maria ^{[5
]}

Donato, Hugo ^{[2
,5
]}

机构：

[1] Hosp Nacl Alejandro Posadas, Div Hematol Clin, Dept Med, RA-1419 Buenos Aires, DF, Argentina

[2] Univ Buenos Aires, Fac Ciencias Exactas & Nat, Dept Quim Biol, Lab Anal Biol, Buenos Aires, DF, Argentina

[3] Consultorios Hematol Infantil, Buenos Aires, DF, Argentina

[4] Hosp Nacl Alejandro Posadas, Dept Diagnost, Lab Citometria, RA-1419 Buenos Aires, DF, Argentina

[5] Hosp Municipal Nino de San Justo, Secc Hematol Oncol, Buenos Aires, DF, Argentina

来源：

PEDIATRIC BLOOD & CANCER | 2012年 / 59卷 / 07期

关键词：

capillary blood; flow cytometry; hereditary spherocytosis; hypertonic cryohemolysis; neonatal anemia; osmotic fragility; NEWBORN;

D O I：

10.1002/pbc.24157

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

We studied 31 children with hemolytic anemia, or with positive family history for hereditary spherocytosis (HS), to assess the reliability of capillary blood samples for the diagnosis. HS was diagnosed in 20 patients. Cryohemolysis (CH) was positive in 94% and eosin-5'-maleimide flow cytometry in 90% of them, whereas flow cytometric osmotic fragility was positive in 94%. Capillary blood sampling showed to be useful for the diagnosis. Simultaneous use of these three tests allows confirming diagnosis in 100% of patients. The use of very small blood volumes (300 mu l) allows an earlier diagnosis in neonates and small infants. Pediatr Blood Cancer 2012; 59: 12991301. (C) 2012 Wiley Periodicals, Inc.

引用

页码：1299 / 1301

页数：3

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