Immobilized metal affinity chromatography of human growth hormone - Effect of ligand density

被引：49

作者：

Liesiene, J ^{[1
]}

Racaityte, K ^{[1
]}

Morkeviciene, M ^{[1
]}

Valancius, P ^{[1
]}

Bumelis, V ^{[1
]}

机构：

[1] UAB BIOFA, LT-2028 VILNIUS, LITHUANIA

来源：

JOURNAL OF CHROMATOGRAPHY A | 1997年 / 764卷 / 01期

关键词：

immobilized metal affinity chromatography; ligand density; growth hormones; somatotropin; proteins;

D O I：

10.1016/S0021-9673(96)00869-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Immobilized metal affinity chromatography was examined as a method for the purification of recombinant human growth hormone, somatotropin. Cellulose-based chelating supports, Chelat-Granocel, of a different content of ligand, charged with Cu(II), were assessed for their ability to bind the protein from both crude extract and solution purified by two chromatography steps. Human growth hormone was found to exhibit high affinity to chelating support charged with Cu(II). One step of immobilized metal affinity chromatography on Chelat-Granocel gives 80% purification of the protein. It was shown that the protein retention depends highly on the ligand density. By regulating sorbent ligand density a favourable desorption was achieved.

引用

页码：27 / 33

页数：7

共 13 条

[1]

ANDERSSON L, 1987, CANCER RES, V47, P3624

[2]

FLODH H, 1986, ACTA PAEDIATR SCAND, P1

[3] MATURATION OF HEAD OF BACTERIOPHAGE-T4 .1. DNA PACKAGING EVENTS [J].