Nucleic acid amplification-based techniques for pathogen detection and identification

被引：106

作者：

Monis, PT ^{[1
]}

Giglio, S ^{[1
]}

机构：

[1] S Australian Water Corp, Australian Water Qual Ctr, Microbiol Unit, Salisbury, SA 5108, Australia

来源：

INFECTION GENETICS AND EVOLUTION | 2006年 / 6卷 / 01期

关键词：

nucleic acid; amplification; detection; real-time PCR; PCR; NASBA; biosensor; LCR;

D O I：

10.1016/j.meegid.2005.08.004

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Nucleic acid amplification techniques have revolutionised diagnostic and research industries. Current technologies that allow the detection of amplification in real-time are fast becoming industry standards, particularly in a diagnostic context. In this review, we describe and explore the application of numerous real-time detection chemistries and amplification techniques for pathogen detection and identification, including the polymerase chain reaction, nucleic acid sequence-based amplification, strand displacement amplification and the ligase chain reaction. The emergence of newer technologies, Such as lab-on-a-chip devices and photo-cleavable linkers, is also discussed. Crown Copyright (c) 2005 Published by Elsevier B.V. All rights reserved.

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页码：2 / 12

页数：11

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