Characterization of the dystrophin-syntrophin interaction using the two-hybrid system in yeast

被引：14

作者：

Castello, A ^{[1
]}

Brocheriou, V ^{[1
]}

Chafey, P ^{[1
]}

Kahn, A ^{[1
]}

Gilgenkrantz, H ^{[1
]}

机构：

[1] INST COCHIN GENET MOLEC,INSERM,U129,F-75014 PARIS,FRANCE

来源：

FEBS LETTERS | 1996年 / 383卷 / 1-2期

关键词：

protein-protein interaction; 59; DAP; Duchenne muscular dystrophy;

D O I：

10.1016/0014-5793(96)00214-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The carboxy-terminal region of dystrophin has previously been shown to interact directly with alpha 1 syntrophin, a cytoplasmic component of the dystrophin-glycoprotein complex, by in vitro biochemical studies such as overlay assay or immunoprecipitation. Using the two-hybrid system, we have isolated from a human heart cDNA library the entire coding sequence of human al syntrophin, therefore confirming for the first time this interaction via an in vivo approach. In addition, we have reduced the interaction domain to the distal half of oil syntrophin.

引用

页码：124 / 128

页数：5

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