Quantitative determination of cytotoxic friedo-nor-oleanane derivatives from five morphological types of Maytenus ilicifolia (Celastraceae) by reverse-phase high-performance liquid chromatography

被引：36

作者：

Buffa, W

Corsino, J

Bolzani, VD

Furlan, M

Pereira, AMS

França, SC

机构：

[1] Univ Estadual Paulista, Inst Quim, BR-14801970 Araraquara, SP, Brazil

[2] UNAERP, Dept Biotechnol, BR-14096380 Ribeirao Preto, Brazil

来源：

PHYTOCHEMICAL ANALYSIS | 2002年 / 13卷 / 02期

关键词：

reverse-phase high-performance liquid chromatography; quantitative determination; friedo-nor-oleanane derivatives; root bark; Maytenus ilicifolia; Celastraceae; morphological types;

D O I：

10.1002/pca.626

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Five different morphological types of Maytenus ilicifolia of the same age and harvested under the same conditions showed distinct accumulations of some friedo-nor-oleananes. A rapid, sensitive and reliable reverse-phase HPLC method (employing an external standard) was used for the determination of the cytotoxic triterpenoids, 20alpha-hydroxymaytenin, 22beta-hydroxymaytenin, maytenin, celastrol and pristimerin in each of the five types. Well resolved peaks with good detection response and linearity in the range 1.0-100 mug/mL were obtained. Copyright (C) 2002 John Wiley Sons, Ltd.

引用

页码：75 / 78

页数：4

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