Inhibition of angiotensin converting enzyme and potentiation of bradykinin by retro-inverso analogues of short peptides and sequences related to angiotensin I and bradykinin

被引:13
作者
Carmona, AK
Juliano, L
机构
[1] Department of Biophysics, Escola Paulista de Medicina, São Paulo, SP
[2] Department of Biophysics, Escola Paulista de Medicina, São Paulo 04044-020, SP
关键词
retro-inverso peptide; bradykinin potentiation; angiotensin converting enzyme inhibitors; peptide synthesis;
D O I
10.1016/0006-2952(96)00047-0
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
There is pharmacological evidence indicating that, in addition to the inhibition of angiotensin converting enzyme (ACE; EC 3.4.15.1), the potentiation of bradykinin (BK) responses may also involve the BK receptor or some binding site in the structures involved in the contractile response to this peptide. Dipeptides such as Val-Trp and some of its analogues as well as tripeptide homologues, including total and partial retroinverso peptides, were synthesized and assayed for their ability to inhibit purified guinea pig plasma ACE and to potentiate the action of BK on the isolated ileum of the same species. The peptides containing the P-2-P-1, P-1-P-1', and P-1'-P-2' inverted amide bonds inhibited ACE, were resistant to hydrolysis, and, depending on the amino acid composition, some of them potentiated the contractile response to BK while others did not. Des-[Arg(1)]-BK, which has an intrinsic activity at concentrations higher than 10(-5) M, and the very dissimilar angiotensin I (AI) analogue [Cys(5)-Cys(10)]-angiotensin-I-(5-10)-amide, which has no detectable contractile activity, were able to inhibit ACE and potentiate BK. In contrast to these peptides, BPP5a and BPP9a from Bothrops jararaca venom, and Potentiators B and C from Agkistrodon halys blomhoffii venom were more effective as BK potentiators than as ACE inhibitors. In conclusion, we have synthesized and assayed compounds that preferentially inhibit ACE, e.g. retro-inverso tripeptides, or potentiate the response of smooth muscle to BK, e.g. snake venom peptides.
引用
收藏
页码:1051 / 1060
页数:10
相关论文
共 46 条
[1]  
ANTONACCIO MJ, 1979, P SOC EXP BIOL MED, V162, P429
[2]   ACE INHIBITORS ARE ENDOTHELIUM DEPENDENT VASODILATORS OF CORONARY-ARTERIES DURING SUBMAXIMAL STIMULATION WITH BRADYKININ [J].
AUCHSCHWELK, W ;
BOSSALLER, C ;
CLAUS, M ;
GRAF, K ;
GRAFE, M ;
FLECK, E .
CARDIOVASCULAR RESEARCH, 1993, 27 (02) :312-317
[3]  
BENDHACK LM, 1986, HYPERTENSION, V8, P90
[4]  
Bergmann M, 1936, J BIOL CHEM, V113, P341
[5]  
BONELLI F, 1984, INT J PEPT PROT RES, V24, P553
[6]  
CAMARGO A, 1971, BRIT J PHARMACOL, V42, P305
[7]   EFFECTS OF CONVERTING-ENZYME INHIBITORS ON ANGIOTENSIN AND BRADYKININ PEPTIDES [J].
CAMPBELL, DJ ;
KLADIS, A ;
DUNCAN, AM .
HYPERTENSION, 1994, 23 (04) :439-449
[8]   SUBSTRATE SPECIFICITIES OF TISSUE KALLIKREIN AND T-KININOGENASE - THEIR POSSIBLE ROLE IN KININOGEN PROCESSING [J].
CHAGAS, JR ;
HIRATA, IY ;
JULIANO, MA ;
XIONG, W ;
WANG, C ;
CHAO, J ;
JULIANO, L ;
PRADO, ES .
BIOCHEMISTRY, 1992, 31 (21) :4969-4974
[9]  
CHEUNG HS, 1980, J BIOL CHEM, V255, P401
[10]   RETRO-INVERSO ISOMERIZATION OF PEPTIDES - SIDE REACTIONS IN THE SYNTHESIS OF N,N'-DIACYL-1,1-DIAMINO-2-PHENYLETHANE DERIVATIVES [J].
CHOREV, M ;
MACDONALD, SA ;
GOODMAN, M .
JOURNAL OF ORGANIC CHEMISTRY, 1984, 49 (05) :821-827