Presence of pathogenic Vibrio Parahaemolyticus in waters and seafood from the Tunisian Sea

被引:9
作者
Khouadja, Sadok [1 ]
Suffredini, Elisabetta [2 ]
Spagnoletti, Matteo [3 ]
Croci, Luciana [2 ]
Colombo, Mauro M. [3 ]
Amina, Bakhrouf [1 ]
机构
[1] Fac Pharm, Dept Microbiol, Lab Anal Traitement & Valorisat Polluants Environ, Monastir 5000, Tunisia
[2] Ist Super Sanita, Dipartimento Sanita Pubbl Vet & Sicurezza Aliment, Reparto Adempimenti Comunitari & Sanita Pubbl, I-00161 Rome, Italy
[3] Univ Roma La Sapienza, Dipartimento Biol & Biotecnol Charles Darwin, I-00185 Rome, Italy
关键词
Vibrio parahaemolyticus; toxR gene; tdh and trh genes; Virulence properties; POLYMERASE-CHAIN-REACTION; DIRECT HEMOLYSIN GENE; UNITED-STATES; COASTAL AREAS; TDH-GENE; IDENTIFICATION; STRAINS; TRH; OYSTERS; FRANCE;
D O I
10.1007/s11274-013-1297-1
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The occurrence of the hemolysin genes, tdh and trh, in Vibrio parahaemolyticus strains isolated from environmental samples collected from various exported seafood products comprising of fishes and shellfish (Mytilus edulis and Crassostrea gigas) or seawater, was studied. Eight strains were confirmed as V. parahaemolyticus by toxR -based polymerase chain reaction and only one strain out of these 8 strains was positive for tdh and trh genes. Toxigenic V. parahaemolyticus isolates are present in Tunisian coastal areas and they may also be present in Tunisian exported seafood products.
引用
收藏
页码:1341 / 1348
页数:8
相关论文
共 34 条
[1]   Isolation and characterization of Vibrio parahaemolyticus causing infection in Iberian toothcarp Aphanius iberus [J].
Alcaide, E ;
Amaro, C ;
Todolí, R ;
Oltra, R .
DISEASES OF AQUATIC ORGANISMS, 1999, 35 (01) :77-80
[2]   A SET OF KEYS FOR BIOCHEMICAL-IDENTIFICATION OF ENVIRONMENTAL VIBRIO SPECIES [J].
ALSINA, M ;
BLANCH, AR .
JOURNAL OF APPLIED BACTERIOLOGY, 1994, 76 (01) :79-85
[3]   Detection of total and hemolysin-producing Vibrio parahaemolyticus in shellfish using multiplex PCR amplification of tl, tdh and trh [J].
Bej, AK ;
Patterson, DP ;
Brasher, CW ;
Vickery, MCL ;
Jones, DD ;
Kaysner, CA .
JOURNAL OF MICROBIOLOGICAL METHODS, 1999, 36 (03) :215-225
[5]   Comparison of different biochemical and molecular methods for the identification of Vibrio parahaemolyticus [J].
Croci, L. ;
Suffredini, E. ;
Cozzi, L. ;
Toti, L. ;
Ottaviani, D. ;
Pruzzo, C. ;
Serratore, P. ;
Fischetti, R. ;
Goffredo, E. ;
Loffredo, G. ;
Mioni, R. .
JOURNAL OF APPLIED MICROBIOLOGY, 2007, 102 (01) :229-237
[6]  
Croci L, 2007, J AOAC INT, V90, P1588
[7]   INCIDENCE OF VIBRIO-PARAHAEMOLYTICUS IN UNITED-STATES COASTAL WATERS AND OYSTERS [J].
DEPAOLA, A ;
HOPKINS, LH ;
PEELER, JT ;
WENTZ, B ;
MCPHEARSON, RM .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1990, 56 (08) :2299-2302
[8]   Seasonal abundance of total and pathogenic Vibrio parahaemolyticus in Alabama oysters [J].
DePaola, A ;
Nordstrom, JL ;
Bowers, JC ;
Wells, JG ;
Cook, DW .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2003, 69 (03) :1521-1526
[9]   Application of polymerase chain reaction for detection of Vibrio parahaemolyticus associated with tropical seafoods and coastal environment [J].
Dileep, V ;
Kumar, HS ;
Kumar, Y ;
Nishibuchi, M ;
Karunasagar, I ;
Karunasagar, I .
LETTERS IN APPLIED MICROBIOLOGY, 2003, 36 (06) :423-427
[10]  
FDA F Drug Administration, 1992, BACTERIOLOGICAL ANAL