Expression profiles and function of Toll-like receptors 2 and 4 in peripheral blood mononuclear cells of chronic hepatitis B patients

被引：173

作者：

Chen, Zhiao ^{[1
,2
]}

Cheng, Yuming ^{[1
,2
]}

Xu, Yongfen ^{[2
,3
]}

Liao, Jing ^{[2
]}

Zhang, Xiaonan ^{[2
]}

Hu, Yunwen ^{[2
]}

Zhang, Qin ^{[2
]}

Wang, Jiefei ^{[2
]}

Zhang, Zhanqing ^{[2
]}

Shen, Fang ^{[2
]}

Yuan, Zhenghong ^{[1
,2
,3
]}

机构：

[1] Fudan Univ, Shanghai Med Coll, Mol Virol Lab, Shanghai 200032, Peoples R China

[2] Fudan Univ, Shanghai Publ Hlth Clin Ctr, Res Unit, Shanghai 201508, Peoples R China

[3] Fudan Univ, Inst Med Microbiol & Biomed Sci, Shanghai 200032, Peoples R China

来源：

CLINICAL IMMUNOLOGY | 2008年 / 128卷 / 03期

基金：

中国国家自然科学基金;

关键词：

Toll-like receptors; hepatitis B virus; peripheral blood mononuclear cells; cytokine;

D O I：

10.1016/j.clim.2008.04.006

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Toll-like receptors (TLRs) play a central role in sensing and initiating innate antiviral response. In this study, we first investigated the expression of TLR1-10 mRNA transcripts in peripheral blood mononuclear cells (PBMCs) from chronic HBV-infected (CHB) patients and healthy donors by quantitative real-time PCR. The expression of TLR1, TLR2, TLR4 and TLR6 transcripts was significantly tower in PBMCs from CHB patients, and the downregulation of TLR2 was related to HBV genotype C. Flow cytometric analysis showed that the expression of TLR2 on PBMCs was significantly decreased in CHB patients. Furthermore, impaired cytokine production was observed in PBMCs from CHB patients after challenged with TLR2 and TLR4 ligands and was correlated with the levels of plasma hepatitis B virus surface antigen (HBsAg). In conclusion, our study reveals a possible interaction between HBsAg, TLR signaling and the innate immune response, which may partially explain the mechanism of HBV infection induced immuno-tolerance. (C) 2008 Elsevier Inc. Alt rights reserved.

引用

页码：400 / 408

页数：9

共 27 条

[1] Tumor necrosis factor alpha inhibition of hepatitis B virus replication involves disruption of capsid integrity through activation of NF-κB [J].

Biermer, M ;

Puro, R ;

Schneider, RJ .

JOURNAL OF VIROLOGY, 2003, 77 (07) :4033-4042

[2] Innate sensing of viruses by toll-like receptors [J].

Boehme, KW ;

Compton, T .

JOURNAL OF VIROLOGY, 2004, 78 (15) :7867-7873

[3]

FERRARI C, 1990, J IMMUNOL, V145, P3442

[4] Mechanisms of disease: Hepatitis B virus infection - Natural history and clinical consequences [J].

Ganem, D ;

Prince, AM .

NEW ENGLAND JOURNAL OF MEDICINE, 2004, 350 (11) :1118-1129

[5] TLR9 expression and function is abolished by the cervical cancer-associated human papillomavirus type 16 [J].

Hasan, Uzma A. ;

Bates, Elizabeth ;

Takeshita, Fumihiko ;

Biliato, Alexandra ;

Accardi, Rosita ;

Bouvard, Veronique ;

Mansour, Mariam ;

Vincent, Isabelle ;

Gissmann, Lutz ;

Iftner, Thomas ;

Sideri, Mario ;

Stubenrauch, Frank ;

Tommasino, Massimo .

JOURNAL OF IMMUNOLOGY, 2007, 178 (05) :3186-3197

[6] Toll-like receptor 3 is induced by and mediates antiviral activity against rhinovirus infection of human bronchial epithelial cells [J].

Hewson, CA ;

Jardine, A ;

Edwards, MR ;

Laza-Stanca, V ;

Johnston, SL .

JOURNAL OF VIROLOGY, 2005, 79 (19) :12273-12279

[7] Expression and regulation of the pattern recognition receptors Toll-like receptor-2 and Toll-like receptor-4 in the human placenta [J].

Holmlund, U ;

Cebers, G ;

Dahlfors, AR ;

Sandstedt, B ;

Bremme, K ;

Ekström, ES ;

Scheynius, A .

IMMUNOLOGY, 2002, 107 (01) :145-151

[8] Quantitative expression of Toll-like receptor 1-10 mRNA in cellular subsets of human peripheral blood mononuclear cells and sensitivity to CpG oligodeoxynucleotides [J].

Hornung, V ;

Rothenfusser, S ;

Britsch, S ;

Krug, A ;

Jahrsdörfer, B ;

Giese, T ;

Endres, S ;

Hartmann, G .

JOURNAL OF IMMUNOLOGY, 2002, 168 (09) :4531-4537

[9]

Hui CK, 2005, HEPATOLOGY, V42, p707A

[10]

Janeway CA, 2005, Immunobiology: the immune system in health and disease

← 1 2 3 →