Midgut Microbiota of the Malaria Mosquito Vector Anopheles gambiae and Interactions with Plasmodium falciparum Infection

被引:362
作者
Boissiere, Anne [1 ]
Tchioffo, Majoline T. [1 ,2 ]
Bachar, Dipankar [3 ]
Abate, Luc [1 ]
Marie, Alexandra [1 ]
Nsango, Sandrine E. [1 ,2 ,4 ]
Shahbazkia, Hamid R. [3 ,5 ]
Awono-Ambene, Parfait H. [2 ]
Levashina, Elena A. [4 ]
Christen, Richard [3 ]
Morlais, Isabelle [1 ,2 ]
机构
[1] UMR MIVEGEC IRD 224 CNRS 5290 UM1 UM2, Montpellier, France
[2] IRD OCEAC, Lab Rech Paludisme, Yaounde, Cameroon
[3] Univ Nice Sophia Antipolis, UMR Systemat Adaptat Evolut 7138, Parc Valrose, France
[4] Univ Strasbourg, CNRS UPR 9022, Inserm U963, Strasbourg, France
[5] Univ Algarve, Fac Ciencias & Tecnol, Area Dept Engn Elect & Comp, Faro, Portugal
关键词
MOLECULAR-FORMS; SPOROGONIC DEVELOPMENT; BACTERIAL DIVERSITY; GUT MICROBIOTA; INTESTINAL MICROBIOTA; DIPTERA; COMMUNITIES; CULICIDAE; BURKHOLDERIA; ENVIRONMENT;
D O I
10.1371/journal.ppat.1002742
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The susceptibility of Anopheles mosquitoes to Plasmodium infections relies on complex interactions between the insect vector and the malaria parasite. A number of studies have shown that the mosquito innate immune responses play an important role in controlling the malaria infection and that the strength of parasite clearance is under genetic control, but little is known about the influence of environmental factors on the transmission success. We present here evidence that the composition of the vector gut microbiota is one of the major components that determine the outcome of mosquito infections. A. gambiae mosquitoes collected in natural breeding sites from Cameroon were experimentally challenged with a wild P. falciparum isolate, and their gut bacterial content was submitted for pyrosequencing analysis. The meta-taxogenomic approach revealed a broader richness of the midgut bacterial flora than previously described. Unexpectedly, the majority of bacterial species were found in only a small proportion of mosquitoes, and only 20 genera were shared by 80% of individuals. We show that observed differences in gut bacterial flora of adult mosquitoes is a result of breeding in distinct sites, suggesting that the native aquatic source where larvae were grown determines the composition of the midgut microbiota. Importantly, the abundance of Enterobacteriaceae in the mosquito midgut correlates significantly with the Plasmodium infection status. This striking relationship highlights the role of natural gut environment in parasite transmission. Deciphering microbe-pathogen interactions offers new perspectives to control disease transmission.
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页数:12
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