Red light activates a chloroplast-dependent ion uptake mechanism for stomatal opening under reduced CO2 concentrations in Vicia spp.

被引:57
作者
Olsen, RL
Pratt, RB
Gump, P
Kemper, A
Tallman, G
机构
[1] Willamette Univ, Dept Biol, Salem, OR 97301 USA
[2] Pepperdine Univ, Div Nat Sci, Malibu, CA 90265 USA
关键词
CO2; guard cell chloroplasts; guard cells; red light transduction; stomata;
D O I
10.1046/j.0028-646X.2001.00337.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Under red light in ambient CO2 guard cells of faba bean (Vicia faba) fix CO2 and accumulate sucrose, causing stomata to open. We examined whether at [CO2] low enough to limit guard cell photosynthesis stomata would open when illuminated with red (R) or far-red (FR) light. After illumination with R or FR in buffered KCl solutions, net stomatal opening was c. 3 mum (R and FR) in air containing 210-225 mul l(-1) CO2 and was 5 mum (R) or 6.5 mum (FR) in air containing 40-50 mul l(-1) CO2. Opening was fully inhibited by 3-(3,4-dichlorophenyl)-1,1 dimethyl urea, the calmodulin antagonist W-7, the ser/thr kinase inhibitor ML-9, and sodium orthovanadate, but not by dithiothreitol, which inhibits formation of zeaxanthin, the blue light photoreceptor of guard cells. Stomatal opening was accompanied by K+ uptake and starch loss. Similar results were obtained when leaves were exposed to conditions designed to lower intercellular leaf [CO2]. These data suggest that the guard cell chloroplasts transduce reduced [CO2], activating stomatal opening through an ion uptake mechanism that depends on chloroplastic photosynthetic electron transport and that shares downstream components of the blue light signal transduction cascade.
引用
收藏
页码:497 / 508
页数:12
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