Mesenchymal Stem Cells as a Gene Delivery System to Create Biological Pacemaker Cells in vitro

被引：13

作者：

Yang, X-J ^{[1
]}

Zhou, Y. F. ^{[1
]}

Li, H-X ^{[1
]}

Han, L-H ^{[1
]}

Jiang, W-P ^{[1
]}

机构：

[1] Soochow Univ, Dept Cardiol, Affiliated Hosp 1, Suzhou 215006, Jiangsu Prov, Peoples R China

来源：

JOURNAL OF INTERNATIONAL MEDICAL RESEARCH | 2008年 / 36卷 / 05期

关键词：

MESENCHYMAL STEM CELLS; HYPERPOLARIZATION-ACTIVATED CYCLIC NUCLEOTIDE-GATED CHANNEL; PACEMAKER CELLS; LENTIVIRUS;

D O I：

10.1177/147323000803600523

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Pacemaker cells differ from common cardiomyocytes due to the presence of a spontaneous depolarization process during the diastolic phase of the cardiac cycle. This is due to hyperpolarization-activated cyclic nucleotide-gated (HCN) channels, which are responsible for providing an inward current. Genetically engineered mesenchymal stem cells (MSCs) were transfected with hHCN4 genes using lentiviral transfection, and their potential use as biological pacemaker cells was investigated. In addition to expressing an anticipated high level of the hHCN4 gene, MSCs transfected with hHCN4 genes also expressed characteristic hHCN4 protein, a cardiac pacemaker-like current and were capable of increasing the spontaneous beating rate of co-cultured cardiac myocytes. Control MSCs did not exert these effects. It is hypothesized that genetically engineered MSCs transfected with hHCN4 genes by lentiviral transfection can be modified to be cardiac pacemaker cells in vitro.

引用

页码：1049 / 1055

页数：7

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