Chromosome sites play dual roles to establish homologous synapsis during meiosis in C-elegans

被引:224
作者
Macqueen, AJ [1 ]
Phillips, CM
Bhalla, N
Weiser, P
Villeneuve, AM
Dernburg, AF
机构
[1] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
[2] Stanford Univ, Sch Med, Dept Dev Biol, Stanford, CA 94305 USA
[3] Lawrence Berkeley Lab, Life Sci Div, Berkeley, CA 94720 USA
关键词
D O I
10.1016/j.cell.2005.09.034
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have investigated the role of pairing centers (PCs), cis-acting sites required for accurate segregation of homologous chromosomes during meiosis in C. elegans. We find that these sites play two distinct roles that contribute to proper segregation. Chromosomes lacking PCs usually fail to synapse and also lack a synapsis-independent stabilization activity. The presence of a PC on just one copy of a chromosome pair promotes synapsis but does not support synapsis independent pairing stabilization, indicating that these functions are separable. Once initiated, synapsis is highly processive, even between nonhomologous chromosomes of disparate lengths, elucidating how translocations suppress meiotic recombination in C. elegans. These findings suggest a multistep pathway for chromosome synapsis in which PCs impart selectivity and efficiency through a "kinetic proofreading" mechanism. We speculate that concentration of these activities at one region per chromosome may have coevolved with the loss of a point centromere to safeguard karyotype stability.
引用
收藏
页码:1037 / 1050
页数:14
相关论文
共 37 条
[1]  
Albertson DG, 1997, C ELEGANS, P47
[2]  
[Anonymous], 1973, ANIMAL CYTOLOGY EVOL
[3]  
BARBAZUK WB, 1994, GENETICS, V136, P129
[4]  
CARLTON PM, 2005, IN PRESS PLOS GENET
[5]   Synaptonemal complex assembly in C-elegans is dispensable for loading strand-exchange proteins but critical for proper completion of recombination [J].
Colaiácovo, MP ;
MacQueen, AJ ;
Martinez-Perez, E ;
McDonald, K ;
Adamo, A ;
La Volpe, A ;
Villeneuve, AM .
DEVELOPMENTAL CELL, 2003, 5 (03) :463-474
[6]   A component of C-elegans meiotic chromosome axes at the interface of homolog alignment, synapsis, nuclear reorganization, and recombination [J].
Couteau, F ;
Nabeshima, K ;
Villeneuve, A ;
Zetka, M .
CURRENT BIOLOGY, 2004, 14 (07) :585-592
[7]   Meiotic recombination in C-elegans initiates by a conserved mechanism and is dispensable for homologous chromosome synapsis [J].
Dernburg, AF ;
McDonald, K ;
Moulder, G ;
Barstead, R ;
Dresser, M ;
Villeneuve, AM .
CELL, 1998, 94 (03) :387-398
[8]   Dynamics of homologous chromosome pairing during meiotic prophase in fission yeast [J].
Ding, DQ ;
Yamamoto, A ;
Haraguchi, T ;
Hiraoka, Y .
DEVELOPMENTAL CELL, 2004, 6 (03) :329-341
[9]   LG II balancer chromosomes in Caenorhabditis elegans:: mT1(II;III) and the mln1 set of dominantly and recessively marked inversions [J].
Edgley, ML ;
Riddle, DL .
MOLECULAR GENETICS AND GENOMICS, 2001, 266 (03) :385-395
[10]  
Edgley ML, 1995, METHOD CELL BIOL, V48, P147