Construction of a medium-density horse gene map

被引：26

作者：

Perrocheau, M

Boutreux, V

Chadi, S

Mata, X

Decaunes, P

Raudsepp, T

Durkin, K

Incarnato, D

Iannuzzi, L

Lear, TL

Hirota, K

Hasegawa, T

Zhu, B

de Jong, P

Cribiu, EP

Chowdhary, BP

Guérin, G

机构：

[1] INRA, Ctr Rech Jouy En Josas, Lab Genet Biochim & Cytogenet, Dept Genet Anim, F-78350 Jouy En Josas, France

[2] Texas A&M Univ, Coll Vet Med, Dept Vet Anat & Publ Hlth, College Stn, TX 77843 USA

[3] ISPAAM, Lab Anim Cytogenet & Gene Mapping, Natl Res Council CNR, Naples, Italy

[4] Univ Kentucky, Gluck Equine Res Ctr, Dept Vet Sci, Lexington, KY 40546 USA

[5] Lab Racing Chem, Dept Mol Genet, Utsunomiya, Tochigi 3200851, Japan

[6] Japan Racing Assoc, Mol & Cellular Biol Lab, Equine Res Inst, Utsunomiya, Tochigi 3200856, Japan

[7] Childrens Hosp, Oakland Res Inst, BACPAC Resources, Oakland, CA 94609 USA

来源：

ANIMAL GENETICS | 2006年 / 37卷 / 02期

关键词：

comparative mapping; gene mapping; horse;

D O I：

10.1111/j.1365-2052.2005.01401.x

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

A medium-density map of the horse genome (Equus caballus) was constructed using genes evenly distributed over the human genome. Three hundred and twenty-three exonic primer pairs were used to screen the INRA and the CHORI-241 equine BAC libraries by polymerase chain reaction and by filter hybridization respectively. Two hundred and thirty-seven BACs containing equine gene orthologues, confirmed by sequencing, were isolated. The BACs were localized to horse chromosomes by fluorescent in situ hybridization (FISH). Overall, 165 genes were assigned to the equine genomic map by radiation hybrid (RH) (using an equine RH5000 panel) and/or by FISH mapping. A comparison of localizations of 713 genes mapped on the horse genome and on the human genome revealed 59 homologous segments and 131 conserved segments. Two of these homologies (ECA27/HSA8 and ECA12p/HSA11p) had not been previously identified. An enhanced resolution of conserved and rearranged chromosomal segments presented in this study provides clarification of chromosome evolution history.

引用

页码：145 / 155

页数：11

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