A simple, chisel-assisted mechanical microdissection system for harvesting homogenous plant tissue suitable for the analysis of nucleic acids and proteins

被引:9
作者
Brandt, S
Walz, C
Schad, M
Pavlovic, N
Kehr, J
机构
[1] Tech Univ Darmstadt, Inst Bot, D-64287 Darmstadt, Germany
[2] Max Planck Inst Mol Plant Physiol, Dept Willmitzer, D-14476 Golm, Germany
[3] Eppendorf AG, D-22339 Hamburg, Germany
关键词
mass spectrometry; mechanical microdissection; paraffin embedding; protein sequencing; RT-PCR; single-cell analysis; tissue specificity;
D O I
10.1007/BF02772591
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Many physiological processes are limited to specific tissues or even specific cell types. Analysing entire plants or organs results in averaged data of all cell types contained in the sample; thus, specific metabolic functions cannot be assigned to individual cell types. A higher spatial resolution is required. By microdissecting plant organs, homogeneous material can be obtained. If a suitable amount of material is collected, standard analytical methods can be applied to elucidate cell type-specific processes. The collection of sufficient quantities of homogeneous material can be done by means of mechanical microdissection. This technique is a low-cost alternative to laser-coupled microdissection techniques. Here we describe a protocol for chisel-assisted mechanical microdissection of embedded plant material and demonstrate that the collected material is suitable to obtain nucleic acids and proteins.
引用
收藏
页码:417 / 427
页数:11
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