Enzyme-free amperometric sensing of glucose by using gold nanoparticles

被引:247
作者
Jena, BK [1 ]
Raj, CR [1 ]
机构
[1] Indian Inst Technol, Dept Chem, Kharagpur 721302, W Bengal, India
关键词
analytical methods; electrochemistry; gold; nanotechnology; sensors;
D O I
10.1002/chem.200501051
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
A nonenzymatic electrochemical method is described for the detection of glucose by using gold (Au) nanoparticles self-assembled on a three-dimensional (3D) silicate network obtained by using sol-gel processes. The nanosized Au particles have been self-assembled on the thiol tail groups of the silicate network and enlarged by hydroxylamine. The Au nanoparticles efficiently catalyze the oxidation of glucose at less-positive potential (0.16 V) in phosphate buffer solution (pH 9.2) in the absence of any enzymes or redox mediators. The Au nanoparticle-modified transducer (MPTS-nAuE) was successfully used for the amperometric sensing of glucose and it showed excellent sensitivity with a detection limit of 50 nm. The common interfering agent ascorbate (AA) does not interfere with the detection of glucose. The MPTS-nAuE transducer showed individual voltammetric responses for glucose and AA. This transducer responded linearly to glucose in the range of 0-8 mm and the sensitivity of the transducer was found to be 0.179 nA cm(-2) nm(-1). Excellent reproducibility, and long-term storage and operational stability was observed for this transducer.
引用
收藏
页码:2702 / 2708
页数:7
相关论文
共 58 条
[1]   ELECTROCHEMICAL OXIDATION OF GLUCOSE ON SINGLE-CRYSTAL GOLD SURFACES [J].
ADZIC, RR ;
HSIAO, MW ;
YEAGER, EB .
JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1989, 260 (02) :475-485
[2]   Photonic crystal carbohydrate sensors: Low ionic strength sugar sensing [J].
Asher, SA ;
Alexeev, VL ;
Goponenko, AV ;
Sharma, AC ;
Lednev, IK ;
Wilcox, CS ;
Finegold, DN .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (11) :3322-3329
[3]   ORGANIC-CHEMISTRY WITHIN CERAMIC MATRICES - DOPED SOL-GEL MATERIALS [J].
AVNIR, D .
ACCOUNTS OF CHEMICAL RESEARCH, 1995, 28 (08) :328-334
[4]   Noninvasive continuous monitoring of physiological glucose using a monosaccharide-sensing contact lens [J].
Badugu, R ;
Lakowicz, JR ;
Geddes, CD .
ANALYTICAL CHEMISTRY, 2004, 76 (03) :610-618
[5]   Covalent attachment of osmium complexes to glucose oxidase and the application of the resulting modified enzyme in an enzyme switch responsive to glucose [J].
Battaglini, F ;
Bartlett, PN ;
Wang, JH .
ANALYTICAL CHEMISTRY, 2000, 72 (03) :502-509
[6]   Novel electrochemical interfaces with a tunable kinetic barrier by self-assembling organically modified silica gel and gold nanoparticles [J].
Bharathi, S ;
Nogami, M ;
Ikeda, S .
LANGMUIR, 2001, 17 (01) :1-4
[7]   PULSED AMPEROMETRIC DETECTION OF GLUCOSE IN BIOLOGICAL-FLUIDS AT A SURFACE-MODIFIED GOLD ELECTRODE [J].
BINDRA, DS ;
WILSON, GS .
ANALYTICAL CHEMISTRY, 1989, 61 (22) :2566-2570
[8]   Hydroxylamine seeding of colloidal Au nanoparticles in solution and on surfaces [J].
Brown, KR ;
Natan, MJ .
LANGMUIR, 1998, 14 (04) :726-728
[9]   Seeding of colloidal Au nanoparticle solutions. 2. Improved control of particle size and shape [J].
Brown, KR ;
Walter, DG ;
Natan, MJ .
CHEMISTRY OF MATERIALS, 2000, 12 (02) :306-313
[10]   Scope for new applications for gold arising from the electrocatalytic behaviour of its metastable surface states [J].
Burke, LD .
GOLD BULLETIN, 2004, 37 (1-2) :125-135