Detection by nested-PCR of Toxoplasma gondii in patients infected with human immunodeficiency virus

被引:9
作者
Alonso, R
Martínez, E
Laynez, P
Miguélez, M
Piñero, JE
Valladares, B
机构
[1] Univ La Laguna, Fac Farm, Dept Parasitol, Tenerife 38271, Spain
[2] Hosp Candelaria, Med Interna Serv, Santa Cruz De Tenerife, Spain
来源
MEDICINA CLINICA | 2002年 / 118卷 / 08期
关键词
taxoplasmosis; HIV; nested-PCR;
D O I
10.1016/S0025-7753(02)72363-4
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
BACKGROUND: Our purpose was to evaluate the effectiveness of the nested polymerase chain reaction (nested-PCR) for the laboratory diagnosis of active toxoplasmosis in patients infected with human immunodeficiency virus (HIV). PATIENTS AND METHOD: The study was carried out in 157 HIV-infected patients. We determined IgG, IgM and IgA antibodies against Toxoplasma gondii as well as parasite DNA by nested-PCR in blood samples. RESULTS: IgG antibodies were detected in 56 patients, IgM in 1 and IgA in 5 patients. Eleven patients were found to have DNA of the parasite. CONCLUSIONS: The polymerase chain reaction is a rapid, sensitive and effective technique in the early diagnosis of toxoplasmosis in HIV-infected patients. Its positivity may also point to the beginning of treatment in asymptomatic individuals.
引用
收藏
页码:294 / 296
页数:3
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