Control of dehydrodiferulate cross-linking in pectins from sugar-beet tissues

被引:14
作者
Baydoun, EAH
Pavlencheva, N
Cumming, CM
Waldron, KW
Brett, CT
机构
[1] Univ Glasgow, Plant Sci Grp, Inst Biomed & Life Sci, Glasgow G12 8QQ, Lanark, Scotland
[2] Amer Univ Beirut, Dept Biol, Beirut, Lebanon
[3] Russian Acad Sci, Kazan Inst Biochem & Biophys, Kazan 420503, Russia
[4] Inst Food Res, Norwich NR4 7UA, Norfolk, England
基金
英国生物技术与生命科学研究理事会;
关键词
Beta vulgaris L; Chenopodaciaceae; sugar beets; pectin; cross-linking; ferulate; dehydrodiferulate; hydrogen peroxide; peroxidase;
D O I
10.1016/j.phytochem.2004.02.014
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Pectins were extracted from roots, petioles and leaves of sugar beet, and cross-linked using hydrogen peroxide and peroxidase. The effects on dehydrodiferulate formation were monitored by HPLC and TLC. Dehydrodimers were formed in different proportions to those found in vivo. There was a net loss of around 50% of the phenolic groups (monomers plus dimers) during dimerisation. Gel filtration showed that root and petiole pectin, but not leaf pectin, increased in molecular weight during cross-linking. The effects of varying the cross-linking conditions were investigated, and it was found that hydrogen peroxide concentration was the most important factor in controlling both the type and amount of dehydrodiferulate formed. (C) 2004 Elsevier Ltd. All rights reserved.
引用
收藏
页码:1107 / 1115
页数:9
相关论文
共 32 条
[1]   Generation of the oxidative burst - scavenging for the truth [J].
Bestwick, C ;
Bolwell, P ;
Mansfield, J ;
Nicole, M ;
Wojtaszek, P .
TRENDS IN PLANT SCIENCE, 1999, 4 (03) :88-89
[2]   Isolation and identification of a ferulic acid dehydrotrimer from saponified maize bran insoluble fiber [J].
Bunzel, M ;
Ralph, J ;
Funk, C ;
Steinhart, H .
EUROPEAN FOOD RESEARCH AND TECHNOLOGY, 2003, 217 (02) :128-133
[3]   COLORIMETRIC METHOD FOR DETERMINATION OF SUGARS AND RELATED SUBSTANCES [J].
DUBOIS, M ;
GILLES, KA ;
HAMILTON, JK ;
REBERS, PA ;
SMITH, F .
ANALYTICAL CHEMISTRY, 1956, 28 (03) :350-356
[4]  
Grabber JH, 1998, J SCI FOOD AGR, V77, P193, DOI 10.1002/(SICI)1097-0010(199806)77:2<193::AID-JSFA25>3.0.CO
[5]  
2-A
[6]   Model studies of ferulate-coniferyl alcohol cross-product formation in primary maize walls: Implications for lignification in grasses [J].
Grabber, JH ;
Ralph, J ;
Hatfield, RD .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2002, 50 (21) :6008-6016
[7]   OXIDATIVE CROSS-LINKING OF CHEMICALLY AND ENZYMATICALLY MODIFIED SUGAR-BEET PECTIN [J].
GUILLON, F ;
THIBAULT, JF .
CARBOHYDRATE POLYMERS, 1990, 12 (04) :353-374
[8]   Characterization and oxidative cross-linking of sugar beet pectins after mild acid hydrolysis and arabanases and galactanases degradation [J].
Guillon, F. ;
Thibault, J. F. .
FOOD HYDROCOLLOIDS, 1987, 1 (5-6) :547-549
[9]  
Kroon PA, 1999, J SCI FOOD AGR, V79, P355, DOI 10.1002/(SICI)1097-0010(19990301)79:3&lt
[10]  
355::AID-JSFA255&gt