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Rapid spine delivery and redistribution of AMPA receptors after synaptic NMDA receptor activation

被引：993
作者：
Shi, SH
Hayashi, Y
Petralia, RS
Zaman, SH
Wenthold, RJ
Svoboda, K
Malinow, R
机构：
[1] Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA
[2] NIDODS, Neurochem Lab, NIH, Bethesda, MD 20892 USA
来源：
SCIENCE | 1999年 / 284卷 / 5421期
关键词：
D O I：
10.1126/science.284.5421.1811
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
To monitor changes in alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptor distribution in living neurons, the AMPA receptor subunit GluR1 was tagged with green fluorescent protein (GFP), This protein (GluR1-GFP) was functional and was transiently expressed in hippocampal CA1 neurons, in dendrites visualized with two-photon Laser scanning microscopy or electron microscopy, most of the GluR1-GFP was intracellular, mimicking endogenous GluR1 distribution. Tetanic synaptic stimulation induced a rapid delivery of tagged receptors into dendritic spines as well as clusters in dendrites. These postsynaptic trafficking events required synaptic N-methyl-D-aspartate (NMDA) receptor activation and may contribute to the enhanced AMPA receptor-mediated transmission observed during long-term potentiation and activity-dependent synaptic maturation.
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页码：1811 / 1816
页数：6
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