New insights into the structural characteristics of the arabinogalactan - Protein (AGP) fraction of gum Arabic

被引:143
作者
Mahendran, T. [1 ]
Williams, P. A. [1 ]
Phillips, G. O. [1 ]
Al-Assaf, S. [1 ]
Baldwin, T. C. [2 ]
机构
[1] Glyndwr Univ, Wrexham LL11 2AW, Wales
[2] Wolverhampton Univ, Sch Appl Sci, Wolverhampton WV1 1SB, Wulfruna St, England
关键词
gum arable structure; Acacia senegal; arabinogalactan protein; gel permeation chromatography; multiangle light scattering; wattle blossom model; alkaline hydrolysis; deglycosylation;
D O I
10.1021/jf800849a
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
The structural characteristics of the gum exudate of Acacia senegal (gum arabic) have been investigated by monitoring the composition and physicochemical properties before and after treatment with proteolytic enzyme and various alkaline systems. Molecular mass (M,) and radius of gyration (R-g) measurements were performed using gel permeation chromatography (GPC) coupled to refractive index, UV absorbance, and multiangle light scattering detectors and indicated that the macromolecules present have a compact structure. It was found that treatment with proteolytic enzyme caused the arabinogalactan-protein component (AGP) with average molecular mass similar to 2 x 10(6) Da to degrade, yielding material of molecular mass similar to 4 x 10(5) Da, whereas the bulk of the material corresponding to the protein-deficient arabinogalactan component (AG) with molecular mass 4 x 10(5) remained unaffected. Barium hydroxide was found to hydrolyze the polysaccharide component (AG) itself in addition to the proteinaceous component as demonstrated in control experiments using dextran. However, sodium borohydride/sodium hydroxide treatments were unable to hydrolyze dextran and were assumed to hydrolyze only the proteinaceous component of gum arabic. The AGP component was completely degraded, yielding material of molecular mass similar to 4.5 x 10(4) Da. It has been concluded, therefore, that the enzyme did not fully hydrolyze all of the protein present and that the AGP component of gum arabic consists of carbohydrate blocks of similar to 4.5 x 10(4) Da linked to a polypeptide chain consistent with the wattle blossom structure. Because the AGP was degraded to differing extents using a mild and more severe sodium borohydride/sodium hydroxide treatment, it was concluded that the polysaccharide moieties were linked through both O-serine and O-hydroxyproline residues. The gum arabic sample was deglycosylated by treatment with anhydrous hydrogen fluoride and revealed the presence of two putative core proteins of similar to 3 x 10(4) and similar to 5 x 10(3) Da, respectively, which correspond to proteins of approximately 250 and 45 amino acids in length. A new model for the structure of the AGP component has been proposed.
引用
收藏
页码:9269 / 9276
页数:8
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