Orientational behavior of phospholipid membranes with mastoparan studied by 31P solid state NMR

被引：18

作者：

Hori, Y

Demura, M ^{[1
]}

Niidome, T

Aoyagi, H

Asakura, T

机构：

[1] Hokkaido Univ, Grad Sch Sci, Div Biol Sci, Sapporo, Hokkaido 0600810, Japan

[2] Tokyo Univ Agr & Technol, Dept Biotechnol, Tokyo 1848588, Japan

[3] Nagasaki Univ, Fac Engn, Nagasaki 8528521, Japan

来源：

FEBS LETTERS | 1999年 / 455卷 / 03期

关键词：

mastoparan; nuclear magnetic resonance; membrane orientation; phospholipid; non-lamellar phase;

D O I：

10.1016/S0014-5793(99)00881-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Solid state P-31 NMR spectroscopy was used to study the perturbing effect of the wasp venom peptide mastoparan (MP) on lipid bilayers composed of dimyristoylphosphatidylcholine (DMPC) and dimyristoylphosphatidylglycerol (DMPG). The P-31 chemical shift anisotropy of multilamellar vesicles decreased with increasing peptide concentration, indicating that MP interacts strongly and selectively with the charged DMPG head group, Macroscopically oriented MP-lipid samples between glass plates were studied by P-31 NMR as a function of tilt angle. These spectra showed the coexistence of orientation-dependent lamellar signals as well as an isotropic peak, suggesting that MP can induce non-lamellar phases in DMPC/DMPG membranes. (C) 1999 Federation of European Biochemical Societies.

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页码：228 / 232

页数：5

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