Birth of a healthy infant after conception with round spermatids isolated from cryopreserved testicular tissue

Objective: To report a case of nonobstructive azoospermia in which round spermatids recovered from thawed testicular tissue were used for injection. Design: Case report. Setting: Reproductive Medicine Unit, S.I.S.ME.R. Patient(s): A 33-year-old azoospermic man. Intervention(s): Intracytoplasmic sperm injection with frozen-thawed spermatids. Main Outcome Measure(s): Fertilization, embryo cleavage, pregnancy, and delivery. Result(s): Birth of a healthy, chromosomally normal girl. Conclusion(s): Frozen-thawed testicular round spermatids from a patient with a history of incomplete spermatogenesis can maintain their viability and their capacity to fertilize and to lead to full-term pregnancy. (Fertil Steril(R) 1999;72:539-41. (C) 1999 by American Society for Reproductive Medicine.).