New 3′ elements control Pax6 expression in the developing pretecturn, neural retina and olfactory region

被引：76

作者：

Griffin, C ^{[1
]}

Kleinjan, DA ^{[1
]}

Doe, B ^{[1
]}

van Heyningen, V ^{[1
]}

机构：

[1] Western Gen Hosp, MRC, Human Genet Unit, Edinburgh EH4 2XU, Midlothian, Scotland

来源：

MECHANISMS OF DEVELOPMENT | 2002年 / 112卷 / 1-2期

基金：

英国医学研究理事会;

关键词：

Px6; evolutionary sequence conservation; transgenic analysis; cis-acting element; pufferfish;

D O I：

10.1016/S0925-4773(01)00646-3

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Pax6 is a key transcriptional regulator in eye, olfactory system, forebrain, pituitary cerebellum, spinal cord and pancreas development. Alternative splicing, promoter usage and multiple enhancers regulate the complex Pax6 spatio-temporal expression pattern. Chromosomal rearrangements which abolish PAX6 gene expression have been characterised downstream of the coding region. Through evolutionary sequence comparison and transgenic reporter studies, we have identified a new Pax6 3' cis-regulatory region. This region, C1170 Box 123, contains three distinct modules of human-mouse sequence conservation, while only Box 1 is conserved to Fugu. Both the human and the orthologous Fugu sequence direct similar reporter gene expression in the developing pretectum, neural retina and olfactory region, indicating evolutionary conservation of Pax6 regulatory mechanisms despite the low level of overall sequence conservation. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：89 / 100

页数：12

共 60 条

[1]

[Anonymous], 1994, MANIPULATING MOUSE E

[2] DETECTING CONSERVED REGULATORY ELEMENTS WITH THE MODEL GENOME OF THE JAPANESE PUFFER FISH, FUGU RUBRIPES [J].

APARICIO, S ;

MORRISON, A ;

GOULD, A ;

GILTHORPE, J ;

CHAUDHURI, C ;

RIGBY, P ;

KRUMLAUF, R ;

BRENNER, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (05) :1684-1688

[3] Pax-6 in development and evolution [J].

Callaerts, P ;

Halder, G ;

Gehring, WJ .

ANNUAL REVIEW OF NEUROSCIENCE, 1997, 20 :483-532

[4]

Davis JA, 1996, J NEUROSCI, V16, P5082

[5]

Dellovade TL, 1998, J COMP NEUROL, V402, P402

[6]

Dimanlig PV, 2001, DEVELOPMENT, V128, P4415

[7] Active conservation of noncoding sequences revealed by three-way species comparisons [J].

Dubchak, I ;

Brudno, M ;

Loots, GG ;

Pachter, L ;

Mayor, C ;

Rubin, EM ;

Frazer, KA .

GENOME RESEARCH, 2000, 10 (09) :1304-1306

[8] 2 INDEPENDENT AND INTERACTIVE DNA-BINDING SUBDOMAINS OF THE PAX6 PAIRED DOMAIN ARE REGULATED BY ALTERNATIVE SPLICING [J].

EPSTEIN, JA ;

GLASER, T ;

CAI, JX ;

JEPEAL, L ;

WALTON, DS ;

MAAS, RL .

GENES & DEVELOPMENT, 1994, 8 (17) :2022-2034

[9] ANIRIDIA-ASSOCIATED CYTOGENETIC REARRANGEMENTS SUGGEST THAT A POSITION EFFECT MAY CAUSE THE MUTANT PHENOTYPE [J].

FANTES, J ;

REDEKER, B ;

BREEN, M ;

BOYLE, S ;

BROWN, J ;

FLETCHER, J ;

JONES, S ;

BICKMORE, W ;

FUKUSHIMA, Y ;

MANNENS, M ;

DANES, S ;

VANHEYNINGEN, V ;

HANSON, I .

HUMAN MOLECULAR GENETICS, 1995, 4 (03) :415-422

[10] UCHIDA RAT (RSEY) - A NEW MUTANT RAT WITH CRANIOFACIAL ABNORMALITIES RESEMBLING THOSE OF THE MOUSE SEY MUTANT [J].

FUJIWARA, M ;

UCHIDA, T ;

OSUMIYAMASHITA, N ;

ETO, K .

DIFFERENTIATION, 1994, 57 (01) :31-38

← 1 2 3 4 5 6 →