Measles virus spreads in rat hippocampal neurons by cell-to-cell contact and in a polarized fashion

被引:59
作者
Ehrengruber, MU
Ehler, E
Billeter, MA
Naim, HY
机构
[1] Univ Zurich, Inst Mol Biol, CH-8057 Zurich, Switzerland
[2] Univ Zurich, Brain Res Inst, CH-8057 Zurich, Switzerland
[3] Swiss Fed Inst Technol, Inst Cell Biol, CH-8093 Zurich, Switzerland
关键词
D O I
10.1128/JVI.76.11.5720-5728.2002
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Measles virus (MV) can infect the central nervous system and, in rare cases, causes subacute sclerosing panencephalitis, characterized by a progressive degeneration of neurons. The route of MV transmission in neurons was investigated in cultured rat hippocampal slices by using MV expressing green fluorescent protein. MV infected hippocampal neurons and spread unidirectionally, in a retrograde manner, from CA1 to CA3 pyramidal cells and from there to the dentate gyrus. Spreading of infection depended on cell-to-cell contact and occurred without any detectable release of infectious particles. The role of the viral proteins in the retrograde MV transmission was determined by investigating their sorting in infected pyramidal cells. MV glycoproteins, the fusion protein (F) and hemagglutinin (H), the matrix protein (M), and the phosphoprotein (P), which is part of the viral ribonucleoprotein complex, were all sorted to the dendrites. While M, P, and H proteins remained more intracellular, the F protein localized to prominent, spine-type domains at the surface of infected cells. The detected localization of MV proteins suggests that local microfusion events may be mediated by the F protein at sites of synaptic contacts and is consistent with a mechanism of retrograde transmission of MV infection.
引用
收藏
页码:5720 / 5728
页数:9
相关论文
共 48 条
[1]   EXPRESSION OF DEFECTIVE MEASLES-VIRUS GENES IN BRAIN-TISSUES OF PATIENTS WITH SUBACUTE SCLEROSING PANENCEPHALITIS [J].
BACZKO, K ;
LIEBERT, UG ;
BILLETER, M ;
CATTANEO, R ;
BUDKA, H ;
TERMEULEN, V .
JOURNAL OF VIROLOGY, 1986, 59 (02) :472-478
[2]   CLONAL EXPANSION OF HYPERMUTATED MEASLES-VIRUS IN A SSPE BRAIN [J].
BACZKO, K ;
LAMPE, J ;
LIEBERT, UG ;
BRINCKMANN, U ;
TERMEULEN, V ;
PARDOWITZ, I ;
BUDKA, H ;
COSBY, SL ;
ISSERTE, S ;
RIMA, BK .
VIROLOGY, 1993, 197 (01) :188-195
[3]  
BILLETER MA, 1991, PARAMYXOVIRUSES, P323
[4]   ENTRY AND RELEASE OF MEASLES-VIRUS ARE POLARIZED IN EPITHELIAL-CELLS [J].
BLAU, DM ;
COMPANS, RW .
VIROLOGY, 1995, 210 (01) :91-99
[5]   The role of selective transport in neuronal protein sorting [J].
Burack, MA ;
Silverman, MA ;
Banker, G .
NEURON, 2000, 26 (02) :465-472
[6]  
CARD JP, 1990, J NEUROSCI, V10, P1974
[7]   Measles viruses with altered envelope protein cytoplasmic tails gain cell fusion competence [J].
Cathomen, T ;
Naim, HY ;
Cattaneo, R .
JOURNAL OF VIROLOGY, 1998, 72 (02) :1224-1234
[8]   A matrix-less measles virus is infectious and elicits extensive cell fusion: consequences for propagation in the brain [J].
Cathomen, T ;
Mrkic, B ;
Spehner, D ;
Drillien, R ;
Naef, R ;
Pavlovic, J ;
Aguzzi, A ;
Billeter, MA ;
Cattaneo, R .
EMBO JOURNAL, 1998, 17 (14) :3899-3908
[9]   INVASION OF THE PERIPHERAL NERVOUS SYSTEMS OF ADULT MICE BY THE CVS STRAIN OF RABIES VIRUS AND ITS AVIRULENT DERIVATIVE AVO1 [J].
COULON, P ;
DERBIN, C ;
KUCERA, P ;
LAFAY, F ;
PREHAUD, C ;
FLAMAND, A .
JOURNAL OF VIROLOGY, 1989, 63 (08) :3550-3554
[10]   PHYSIOLOGY AND PHARMACOLOGY OF UNITARY SYNAPTIC CONNECTIONS BETWEEN PAIRS OF CELLS IN AREAS CA3 AND CA1 OF RAT HIPPOCAMPAL SLICE CULTURES [J].
DEBANNE, D ;
GUERINEAU, NC ;
GAHWILER, BH ;
THOMPSON, SM .
JOURNAL OF NEUROPHYSIOLOGY, 1995, 73 (03) :1282-1294