Inducible nitric oxide synthase is present in the rat placenta at the fetal-maternal interface and decreases prior to labour

被引:60
作者
Purcell, TL
Buhimschi, IA
Given, R
Chwalisz, K
Garfield, RE
机构
[1] UNIV TEXAS,MED BRANCH,DEPT OBSTET & GYNAECOL,GALVESTON,TX 77555
[2] UNIV TEXAS,MED BRANCH,DEPT ANAT & NEUROSCI,GALVESTON,TX 77555
[3] SCHERING AG,RES LABS,D-1000 BERLIN,GERMANY
关键词
immunohistochemistry; labour; nitric oxide; placenta; Western blot;
D O I
10.1093/molehr/3.6.485
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The aim of this study was to investigate the expression and distribution patterns of the inducible isoform of nitric oxide synthase (iNOS) in rat placenta during gestation and term labour. The expression of iNOS isoform was assessed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting with monoclonal antibodies. Two specific bands were detected corresponding to 135 and 124 kDa in all placenta samples. The upper band (135 kDa) was identified as iNOS due to its correspondence with the band obtained with mouse macrophages (positive control). Compared with its concentrations on day 16, iNOS decreased steadily toward the end of gestation to similar to 37% on day 20, 20% on day 22 before labour and 12% during labour (P < 0.01). The lower band (124 kDa) drastically increased (to almost double) from day 16 to day 18 but returned to initial values on day 22, during delivery. Immunohistochemical staining of placentae at day 16 and 22 using rabbit polyclonal anti-iNOS antibody revealed labelling specifically concentrated in the trophospongial cell layer, at the fetal-maternal interface. The most conspicuous [NOS staining was associated with islands of cells referred to as vacuolated 'glycogen cells). Staining was greatly decreased during labour. The changes in placental iNOS expression suggest a 'paracrine' role for NO in regulating uterine contractility, blood flow and immunosuppression required for pregnancy maintenance. NO withdrawal al term may also be involved in the initiation of labour.
引用
收藏
页码:485 / 491
页数:7
相关论文
共 36 条
[1]   NITRIC-OXIDE - AN UBIQUITOUS MESSENGER [J].
BERDEAUX, A .
FUNDAMENTAL & CLINICAL PHARMACOLOGY, 1993, 7 (08) :401-411
[2]  
BUHIMSCHI C, 1997, IN PRESS AM J OBSTET
[3]   INVOLVEMENT OF A NITRIC OXIDE-CYCLIC GUANOSINE-MONOPHOSPHATE PATHWAY IN CONTROL OF HUMAN UTERINE CONTRACTILITY DURING PREGNANCY [J].
BUHIMSCHI, I ;
YALLAMPALLI, C ;
DONG, YL ;
GARFIELD, RE .
AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY, 1995, 172 (05) :1577-1584
[4]   PRE-ECLAMPSIA-LIKE CONDITIONS PRODUCED BY NITRIC-OXIDE INHIBITION - EFFECTS OF L-ARGININE, D-ARGININE AND STEROID-HORMONES [J].
BUHIMSCHI, I ;
YALLAMPALLI, C ;
CHWALISZ, K ;
GARFIELD, RE .
HUMAN REPRODUCTION, 1995, 10 (10) :2723-2730
[5]  
Buhimschi I, 1996, HUM REPROD, V11, P1755
[6]  
CHWALISZ K, 1997, PRENAT NEONAT MED, V1, P292
[7]  
DAVIES J, 1968, ACTA ANAT, V69, P542
[8]  
DEANE HW, 1961, ENDOCRINOLOGY, V70, P470
[9]  
DiIulio JL, 1995, REPROD FERT DEVELOP, V7, P1505
[10]   CONTROL OF VASCULAR REACTIVITY IN PREGNANCY [J].
GANT, NF ;
WHALLEY, PJ ;
EVERETT, RB ;
WORLEY, RJ ;
MACDONALD, PC .
AMERICAN JOURNAL OF KIDNEY DISEASES, 1987, 9 (04) :303-307