The virulence of Enterococcus to freshwater prawn Macrobrachium rosenbergii and its immune resistance under ammonia stress

被引:132
作者
Cheng, W
Chen, JC [1 ]
机构
[1] Natl Taiwan Ocean Univ, Dept Aquaculture, Chilung 202, Taiwan
[2] Natl Pingtung Univ Sci & Technol, Dept Aquaculture, Pingtung 912, Taiwan
关键词
Macrobrachium rosenbergii; Enterococcus; ammonia; challenge; virulence; haemocyte count; phenoloxidase activity; superoxide anions;
D O I
10.1006/fsim.2001.0363
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
Growth of pathogen bacterium, Enterococcus was not affected in tryptic soy broth (TSB) medium containing ammonia-N concentration in the range of 0-5.14 mg l (1). Giant freshwater prawn Macrobrachium rosenbergii (8-12 g) were challenged with Enterococcus which had been incubated for 24 h in TSB medium containing different concentrations of ammonia-N at 0-5.14 mg l(-1). Cumulative mortality of M. rosenbergii was higher for the bacteria incubated in TSB medium having ammonia-N at 0 and 0.26 mg l(-1), than those incubated in TSB medium having 1.28, 2.57 and 5.14 mg l(-1) ammonia-N after 24 h of challenge. However, cumulative mortality of prawn was significantly higher for the bacteria incubated in TSB medium with no ammonia added after 120 h of challenge. The prawns (8-12 g) were challenged with Enterococcus previously incubated in TSB medium for 24 h, then placed in water having concentrations of ammonia-N at control (0.06 mg l(-1)), 0.55, 1.01, 1.68 and 3.18 mg l(-1). Mortality of prawns increased directly with ammonia-N concentrations after 72 h challenge. The pranws (20 30 g) which had been exposed to control, 0.55,1.68 and 3.18 mg l(-1) ammonia-N for 7 days were examined for the total haemocyte count (THC), differential haemocyte count (DHC), phenoloxidase activity and respiratory burst of haemocytes. Phenoloxidase activity decreased when the prawns were exposed to ammonia-N greater than 0.55 mg l(-1). The respiratory burst increased significantly at 0.55 mg l(-1), but decreased significantly at 1.68 and 3.18 mg l(-1) ammonia-N. No significant difference in haemocyte count was observed among the prawns at different ammonia-N concentrations. It is suggested that ammonia in water decreases the virulence of Enterococcus, and reduces the immune resistance of M. rosenbergii. (C) 2002 Academic Press.
引用
收藏
页码:97 / 109
页数:13
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