Lasp anchors the Drosophila male stem cell niche and mediates spermatid individualization

被引：34

作者：

Lee, Soojin ^{[1
]}

Zhou, Lili ^{[1
]}

Kim, Jieun ^{[1
]}

Kalbfleisch, Stephen ^{[1
]}

Schoeck, Frieder ^{[1
]}

机构：

[1] McGill Univ, Dept Biol, Montreal, PQ H3A 1B1, Canada

来源：

MECHANISMS OF DEVELOPMENT | 2008年 / 125卷 / 9-10期

基金：

加拿大自然科学与工程研究理事会;

关键词：

Lasp; Integrin; Stem cell niche; Cyst cells; Hub cells; Actin cone; Spermatid individualization;

D O I：

10.1016/j.mod.2008.06.012

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Lasp family proteins contain an amino-terminal LIM domain, two actin-binding nebulin repeats and a carboxyl-terminal SH3 domain. Vertebrate Lasp-1 localizes to focal adhesions and the leading edge of migrating cells, and is required for cell migration. To assess the in vivo function of Lasp, we generated a null mutant in Drosophila Lasp. Lasp(1) is homozygous viable, but male sterile. In Lasp mutants the stem cell niche is no longer anchored to the apical tip of the testis, and actin cone migration is perturbed resulting in improper spermatid individualization. Hub cell mislocalization can by phenocopied by expressing Lasp or PPS integrin RNAi transgenes in somatic cells, and Lasp genetically interacts with PPS integrin, demonstrating that Lasp functions together with integrins in hub cells to anchor the stem cell niche. Finally, we show that the stem cell niche is maintained even if it is not properly localized. (C) 2008 Elsevier Ireland Ltd. All rights reserved.

引用

收藏

页码：768 / 776

页数：9

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