Polysaccharide microarrays for polysaccharide-platelet-derived-growth-factor interaction studies

被引：17

作者：

Carion, Olivier

Lefebvre, Julien

Dubreucq, Guy

Dahri-Correia, Latifa

Correia, Jose

Melnyk, Oleg ^{[1
]}

机构：

[1] Univ Lille 1, Biol Inst Lille, UMR 8161, CNRS, 1 Rue Pr Calmette, F-59021 Lille, France

[2] Univ Lille 2, Inst Pasteur Lille, F-59021 Lille, France

[3] BIODEX SA, F-59230 St Amand Les Eaux, France

来源：

CHEMBIOCHEM | 2006年 / 7卷 / 05期

关键词：

dextran; high-throughput screening; microarrays; polysaccharides; proteins;

D O I：

10.1002/cbic.200500387

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Polysaccharide microarrays have great potential for the high-throughput analysis of polysoccharide-protein interactions. Here we demonstrate that a polysaccharide microarray prepared by printing a library of dextran polymers derivatized by methylcarboxylate, benzylamide, and sulfate groups (DMCBSu compounds) on to glass slides permitted the rapid identification of a set of compounds able to interact with the platelet-derived growth factor BB (PDGF-BB) isoform, a growth factor involved in wound heating. Microarray interaction results were compared to the capacity of DMCBSu compounds to potentiate the in vitro PDGF-BB-induced proliferation of human dermal fibroblasts.

引用

收藏

页码：817 / 826

页数：10

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