A genetically encoded photocaged tyrosine

被引：165

作者：

Deiters, A

Groff, D

Ryu, YH

Xie, JM

Schultz, PG

机构：

[1] Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA

[2] Scripps Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92037 USA

[3] N Carolina State Univ, Dept Chem, Raleigh, NC 27695 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2006年 / 45卷 / 17期

关键词：

amino acids; mutagenesis; photocaged tyrosine; proteins;

D O I：

10.1002/anie.200600264

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Chemical Equation Presented) Activating proteins with light: A photo-caged tyrosine was genetically encoded in E. coli in response to the amber codon TAG. Substitution of Tyr503 in the active site of β-galactosidase allowed photoactivation of this enzyme in vitro or directly in bacteria with 360-nm light. This method should allow photoregulation of the activity of a variety of biological processes including transcription, signal transduction, and cellular trafficking. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：2728 / 2731

页数：4

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