Presentation of protective antigen to the mouse immune system: immune sequelae

被引：36

作者：

Williamson, ED ^{[1
]}

Beedham, RJ ^{[1
]}

Bennett, AM ^{[1
]}

Perkins, SD ^{[1
]}

Miller, J ^{[1
]}

Baillie, LWJ ^{[1
]}

机构：

[1] DERA, CBD Sector, Salisbury SP4 0JG, Wilts, England

来源：

JOURNAL OF APPLIED MICROBIOLOGY | 1999年 / 87卷 / 02期

关键词：

D O I：

10.1046/j.1365-2672.1999.00901.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Protective antigen (PA), the major protective component of the existing vaccine, is a potent immunogen. Protective antigen in alhydrogel induced a high serum IgG titre(> log(10) 4) in both the C57B16 and Balb/c mouse and the predominant subclass of antibody induced was IgG1, indicating that the response to PA was predominantly Th, directed. When plasmid DNA encoding PA was used to immunize the Balb/c mouse, a low serum IgG titre was detected (less than or equal to log(10) 1), which was slightly increased by boosting with plasmid DNA. However, when mice immunized with plasmid DNA were later boosted with rPA, a significant and rapid increase in titre (up to threefold) was observed. Priming mice with PA-encoding plasmid DNA may be a mechanism of enhancing and accelerating the immune response to PA.

引用

页码：315 / 317

页数：3

共 6 条

[1] Gene vaccination with naked plasmid DNA: Mechanism of CTL priming [J].