Novel conducting polymer electrolyte biosensor based on poly(1-vinyl imidazole) and poly(acrylic acid) networks

被引：45

作者：

Arslan, A

Kiralp, S

Toppare, L ^{[1
]}

Bozkurt, A

机构：

[1] Middle E Tech Univ, Dept Chem, TR-06531 Ankara, Turkey

[2] Fatih Univ, Dept Chem, TR-34900 Istanbul, Turkey

来源：

LANGMUIR | 2006年 / 22卷 / 06期

关键词：

D O I：

10.1021/la0530539

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Biosensor construction and characterization studies of poly(acrylic acid) (PAA) and poly(1-vinyl imidazole) (PVI) complex systems have been carried out. The biosensors were prepared by mixing PAA with PVI at several stoichiometric ratios, x (molar ratio of the monomer repeat units). The enzyme, invertase, was entrapped in the PAA/PVA interpenetrating polymer networks during complexation. Modifications were made on the PAA/PVI conducting polymer electrolyte matrixes to improve the stability and performance of the polymer electrolyte-based enzyme biosensor. The maximum reaction rate (V-max) and Michaelis-Menten constant (K-m) were investigated for the immobilized invertase. The temperature and pH optimization, operational stability, and shelf life of the polymer electrolyte biosensor were also examined.

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收藏

页码：2912 / 2915

页数：4

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