Conditional and reversible disruption of essential herpesvirus proteins

被引：46

作者：

Glass, Mandy ^{[1
]}

Busche, Andreas ^{[1
]}

Wagner, Karen ^{[1
]}

Messerle, Martin ^{[1
]}

Borst, Eva Maria ^{[1
]}

机构：

[1] Hannover Med Sch, Dept Virol, D-3000 Hannover, Germany

来源：

NATURE METHODS | 2009年 / 6卷 / 08期

关键词：

BACTERIAL ARTIFICIAL CHROMOSOME; NUCLEAR-BODIES; VIRAL GENOME; GENE-PRODUCT; CYTOMEGALOVIRUS; CELLS; VIRUSES; CLONING; MUTANT; IE1;

D O I：

10.1038/nmeth.1346

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Elucidating the function of essential proteins of complex pathogenic viruses is impeded by a paucity of complementing systems. By fusing a destabilizing domain of the FK506-binding protein to essential cytomegalovirus proteins, we generated virus mutants in which amounts of fusion proteins and viral growth can be regulated by the synthetic ligand shield-1. This conditional approach will greatly facilitate the analysis of gene functions of herpesviruses and viruses of other families.

引用

页码：577 / 580

页数：4

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