Optimisation of enzymatic synthesis of cefaclor with in situ product removal and continuous acyl donor feeding

被引：9

作者：

Zhang, Yewang ^{[1
]}

Wei, Dongzhi ^{[1
]}

Li, Dengchao ^{[1
]}

Liu, Shulai ^{[1
]}

Song, Qingxun ^{[1
]}

机构：

[1] E China Univ Sci & Technol, State Key Lab Bioreactor Engn, New World Inst Biotechnol, Shanghai 200237, Peoples R China

来源：

BIOCATALYSIS AND BIOTRANSFORMATION | 2007年 / 25卷 / 01期

关键词：

cefaclor; continuous acyl donor feeding; enzymatic synthesis; in situ product removal; penicillin acylase;

D O I：

10.1080/10242420601141762

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Enzymatic synthesis of cefaclor by penicillin acylase (PA) was carried out under kinetic control with in situ product removal (ISPR). We present a continuous acyl donor feeding strategy for enzymatic reactions. Using this strategy, the conversion of the antibiotic nucleus was improved from 65 to 91%, and the hydrolysis of phenylglycine methyl ester ( PGME) was decreased. Side product ( phenylglycine) production was less than half of that in the control batch. The ratio of synthesis to hydrolysis (S/H) in the process was kept stable for longer and at a higher level than in the control. This is a practical method for enzymatic synthesis of cefaclor.

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收藏

页码：59 / 64

页数：6

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