Stationary phase culture supernatant containing membrane vesicles induced immunity to rainbow trout Oncorhynchus mykiss fry syndrome

被引：34

作者：

Aoki, Masatoshi

Kondo, Motoki

Nakatsuka, Yoshiharu

Kawai, Kenji

Oshima, Syun-Ichirou ^{[1
]}

机构：

[1] Kochi Univ, Grad Sch Kuroshio Sci, Div Marine Bioresource Sci, Lab Cell Struct & Funct,Nanko Ku, Kochi 7838502, Japan

[2] Kochi Univ, Dept Aquaculture, Fish Dis Lab, Kochi 7838502, Japan

来源：

VACCINE | 2007年 / 25卷 / 03期

基金：

日本科学技术振兴机构;

关键词：

Flavobacterium psychrophilum; membrane vesicles (MVs); RTFS; vaccine candidate;

D O I：

10.1016/j.vaccine.2006.07.047

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Flavobacterium psychrophilum is the causative agent of bacterial cold water disease (BCWD) and rainbow trout (Oncorhynchus mykiss) fry syndrome (RTFS). Logarithmic phase formalin-killed cells (FKC) of F. psychrophilum induced immunity to BCWD in ayu (Plecoglossus altivelis) by using an oral administration. In this study, we investigated the effective antigens of logarithmic phase cells in rainbow trout. Rainbow trout fry immunized with logarithmic phase FKC resulted in near complete protection, but the vaccine effect was low in fry immunized with stationary phase FKC. Scanning electron microscopy showed that logarithmic phase cells had many membrane vesicles (MVs) on the surface of F. psychrophilum cells. The MVs were released into medium at the stationary phase. MVs rich supernatant was collected from the stationary phase culture supernatant by using an ammonium precipitation method. Immunization with MVs rich supernatant combined with stationary phase FKC resulted in a relative percentage survival (RPS) of 94-100%, but immunization with MVs rich supernatant only resulted in no protection against F. psychrophilum infection. These data show that MVs have an adjuvant efficacy and suggest that combination of MVs and cells is necessary to obtain efficient protection. (c) 2006 Published by Elsevier Ltd.

引用

页码：561 / 569

页数：9

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