Biotinylated Stealth® magnetoliposomes

被引：31

作者：

Hodenius, M

De Cuyper, M

Desender, L

Müller-Schulte, D

Steigel, A

Lueken, H

机构：

[1] Katholieke Univ Leuven, Interdisciplinary Res Ctr, B-8500 Kortrijk, Belgium

[2] Rhein Westfal TH Aachen, D-52074 Aachen, Germany

[3] Univ Dusseldorf, D-40225 Dusseldorf, Germany

来源：

CHEMISTRY AND PHYSICS OF LIPIDS | 2002年 / 120卷 / 1-2期

关键词：

magnetoliposomes; phospholipid-protein conjugates; phospholipid vesicles; poly(ethylene glycol)-biotin derivatized; phospholipids; streptavidinylated enzymes; Stealth (R) magnetoliposomes;

D O I：

10.1016/S0009-3084(02)00105-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Dimyristoylphosphatidylethanolamine (DC14:0PE) and the dioleoyl analogue (DC18:1cisPE) were mixed with alpha-biotinylamido-omega-N-succinimidoxycarbonyl-poly(ethylene glycol) (NHS-PEG-biotin) and quantitatively converted to alpha-biotinylamido-omega-(dimyristoylphosphatidylethanolamino-carbonyl)polyethylene glycol (DC14:0PE-PEG-biotin) and the dioleoyl analogue DC18:1cisPE-PEG-biotin, respectively. As shown by thin-layer chromatography and H-1 NMR spectroscopy, PEGylation of both phosphatidylethaholamine types went to completion if the reaction was performed in organic solvent in the presence of triethylamine. The resulting derivatives were successfully incorporated into both classical phospholipid vesicles and a phospholipid bilayer surrounding nanometer-sized magnetite cores. In the latter case, the so-called activated Stealth(R)(1) magnetoliposomes were produced which very efficiently immobilized streptavidinylated alkaline phosphatase. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：75 / 85

页数：11

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