Intracellular chloroplast photorelocation in the moss Physcomitrella patens is mediated by phytochrome as well as by a blue-light receptor

被引:69
作者
Kadota, A
Sato, Y
Wada, M
机构
[1] Tokyo Metropolitan Univ, Dept Biol Sci, Grad Sch Sci, Hachioji, Tokyo 1920397, Japan
[2] Natl Inst Basic Biol, Okazaki, Aichi 4448585, Japan
关键词
blue light; chloroplast; photomovement; Physcomitrella (moss); phytochrome;
D O I
10.1007/s004250050700
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The light-induced intracellular relocation of chloroplasts was examined in red-light-grown protonemal cells of the moss Physcomitrella patens. When irradiated with polarized red or blue light, chloroplast distribution in the cell depended upon the direction of the electrical vector (E-vector) in both light qualities. When the E-vector was parallel to the cross-wall (i.e. perpendicular to the protonemal axis), chloroplasts accumulated along the cross-wall; however, no accumulation along the cross-wall was observed when the vector was perpendicular to it (i.e. parallel to the protonemal axis). When a part of the cell was irradiated with a microbeam of red or blue light, chloroplasts accumulated at or avoided the illumination point depending on the fluence rate used. Red light of 0.1-18 W m(-2) and blue light of 0.01-85.5 W m(-2) induced an accumulation response (low-fluence-rate response; LFR), while an avoidance response thigh-fluence-rate response; HFR) was induced by red light of 60 W m(-2) or higher and by blue light of 285 W m(-2). The red-light-induced LFR and HFR were nullified by a simultaneous background irradiation of far-red light, whereas the blue-light-induced LFR and HFR were not affected at all by this treatment. These results show, for the first time, that dichroic phytochrome, as well as the dichroic blue-light receptor, is involved in the chloroplast relocation movement in these bryophyte cells. Further, the phytochrome-mediated responses but not the blue-light responses were revealed to be lost when red-light-grown cells were cultured under white light for 2 d.
引用
收藏
页码:932 / 937
页数:6
相关论文
共 20 条
[1]   ISOLATION AND PRELIMINARY CHARACTERIZATION OF AUXOTROPHIC AND ANALOG RESISTANT MUTANTS OF MOSS, PHYSCOMITRELLA-PATENS [J].
ASHTON, NW ;
COVE, DJ .
MOLECULAR & GENERAL GENETICS, 1977, 154 (01) :87-95
[2]   CONTROL BY PHYTOCHROME OF EXTENSION GROWTH AND POLAROTROPISM IN CHLORONEMATA OF FUNARIA-HYGROMETRICA [J].
BITTISNICH, D ;
WILLIAMSON, RE .
PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1985, 42 (04) :429-436
[3]   The role of mutants in the search for the photoreceptor for phototropism in higher plants [J].
Briggs, WR ;
Liscum, E .
PLANT CELL AND ENVIRONMENT, 1997, 20 (06) :768-772
[4]  
Bunning E., 1958, BER DEUT BOT GES, V71, P304
[5]   GENETIC AND PHYSIOLOGICAL STUDIES OF EFFECT OF LIGHT ON DEVELOPMENT OF MOSS, PHYSCOMITRELLA-PATENS [J].
COVE, DJ ;
SCHILD, A ;
ASHTON, NW ;
HARTMANN, E .
PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1978, 27 (02) :249-254
[6]  
DAVIS BD, 1975, PLANT CELL PHYSIOL, V16, P537
[7]   DER POLAROTROPISMUS UND PHOTOTROPISMUS DER CHLORONEMEN VON DRYOPTERIS FILIX MAS (L) SCHOTT [J].
ETZOLD, H .
PLANTA, 1965, 64 (03) :254-&
[8]   PHYTOCHROME-MEDIATED PHOTOTROPISM IN PROTONEMATA OF THE MOSS CERATODON-PURPUREUS BRID [J].
HARTMANN, E ;
KLINGENBERG, B ;
BAUER, L .
PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1983, 38 (05) :599-603
[9]   CHLOROPLAST MOVEMENT [J].
HAUPT, W ;
SCHEUERLEIN, R .
PLANT CELL AND ENVIRONMENT, 1990, 13 (07) :595-614
[10]   PHOTOTROPISM AND POLAROTROPISM OF PRIMARY CHLORONEMATA OF THE MOSS PHYSCOMITRELLA-PATENS - RESPONSES OF THE WILD-TYPE [J].
JENKINS, GI ;
COVE, DJ .
PLANTA, 1983, 158 (04) :357-364