CD8+ CTL from lungs of Mycobacterium tuberculosis-infected mice express perforin in vivo and Lyse infected macrophages

被引:119
作者
Serbina, NV
Liu, CC
Scanga, CA
Flynn, JL [1 ]
机构
[1] Univ Pittsburgh, Sch Med, Dept Mol Genet & Biochem, Pittsburgh, PA 15206 USA
[2] Univ Pittsburgh, Sch Med, Dept Med, Pittsburgh, PA 15206 USA
[3] Univ Pittsburgh, Sch Med, Div Clin Immunol & Rheumatol, Pittsburgh, PA 15206 USA
关键词
D O I
10.4049/jimmunol.165.1.353
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
CD8(+) T lymphocytes have been implicated in the protective immune response against human and murine tuberculosis. However, the functional role that this cell subset plays during the resolution of infection remains controversial. In this study, we demonstrate the presence of Mycobacterium tuberculosis-specific CD8+ CTL in the lungs and lung-draining lymph nodes of mice infected with dl. tuberculosis via the aerosol or i.v. route. These cells expressed perforin in vivo and specifically recognized and lysed M. tuberculosis-infected macrophages in a perforin-dependent manner after a short period of in vitro restimulation. The efficiency of lysis of infected macrophages was dependent upon the time allowed for interaction between macrophage and M, tuberculosis bacilli. Recognition of infected targets by CD8(+) CTL was beta(2)-microglobulin and MHC class I dependent and was not CD1d restricted. The presented data indicate that CD8(+) T cells contribute to the protective immune response during M. tuberculosis infection by exerting cytotoxic function and lysing infected macrophages.
引用
收藏
页码:353 / 363
页数:11
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