Two-sided ubiquitin binding explains specificity of the TAB2 NZF domain

被引：168

作者：

Kulathu, Yogesh ^{[1
]}

Akutsu, Masato ^{[1
]}

Bremm, Anja ^{[1
]}

Hofmann, Kay ^{[2
]}

Komander, David ^{[1
]}

机构：

[1] MRC, Mol Biol Lab, Cambridge CB2 2QH, England

[2] Miltenyi Biotec, Bergisch Gladbach, Germany

来源：

NATURE STRUCTURAL & MOLECULAR BIOLOGY | 2009年 / 16卷 / 12期

基金：

英国医学研究理事会;

关键词：

KAPPA-B ACTIVATION; POLYUBIQUITIN CHAINS; RECOGNITION; NEMO;

D O I：

10.1038/nsmb.1731

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The protein kinase TAK1 is activated by binding to Lys63 (K63)-linked ubiquitin chains through its subunit TAB2. Here we analyze crystal structures of the TAB2 NZF domain bound to Lys63-linked di- and triubiquitin, revealing that TAB2 binds adjacent ubiquitin moieties via two distinct binding sites. The conformational constraints imposed by TAB2 on a Lys63 dimer cannot be adopted by linear chains, explaining why TAK1 cannot be activated by linear ubiquitination events.

引用

收藏

页码：1328 / 1330

页数：3

相关论文

共 16 条

[1] Ubiquitin interactions of NZF zinc fingers [J].

Alam, SL ;

Sun, J ;

Payne, M ;

Welch, BD ;

Blake, BK ;

Davis, DR ;

Meyer, HH ;

Emr, SD ;

Sundquist, WI .

EMBO JOURNAL, 2004, 23 (07) :1411-1421

[2] TAK1-dependent signaling requires functional interaction with TAB2/TAB3 [J].

Besse, Arnaud ;

Lamothe, Betty ;

Campos, Alejandro D. ;

Webster, William K. ;

Maddineni, Upendra ;

Lin, Su-Chang ;

Wu, Hao ;

Darnay, Bryant G. .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2007, 282 (06) :3918-3928

[3] Nonproteolytic Functions of Ubiquitin in Cell Signaling [J].

Chen, Zhijian J. ;

Sun, Lijun J. .

MOLECULAR CELL, 2009, 33 (03) :275-286

[4] Ubiquitin-binding domains - from structures to functions [J].

Dikic, Ivan ;

Wakatsuki, Soichi ;

Walters, Kylie J. .

NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2009, 10 (10) :659-671

[5] Linear polyubiquitination: a new regulator of NF-κB activation [J].

Iwai, Kazuhiro ;

Tokunaga, Fuminori .

EMBO REPORTS, 2009, 10 (07) :706-713

[6] TAB2 and TAB3 activate the NF-κB pathway through binding to polyubiquitin chains [J].

Kanayama, A ;

Seth, RB ;

Sun, LJ ;

Ea, CK ;

Hong, M ;

Shaito, A ;

Chiu, YH ;

Deng, L ;

Chen, ZJ .

MOLECULAR CELL, 2004, 15 (04) :535-548

[7] The emerging complexity of protein ubiquitination [J].

Komander, David .

BIOCHEMICAL SOCIETY TRANSACTIONS, 2009, 37 :937-953

[8] Molecular discrimination of structurally equivalent Lys 63-linked and linear polyubiquitin chains [J].

Komander, David ;

Reyes-Turcu, Francisca ;

Licchesi, Julien D. F. ;

Odenwaelder, Peter ;

Wilkinson, Keith D. ;

Barford, David .

EMBO REPORTS, 2009, 10 (05) :466-473

[9] Structural Basis for Recognition of Diubiquitins by NEMO [J].

Lo, Yu-Chih ;

Lin, Su-Chang ;

Rospigliosi, Carla C. ;

Conze, Dietrich B. ;

Wu, Chuan-Jin ;

Ashwell, Jonathan D. ;

Eliezer, David ;

Wu, Hao .

MOLECULAR CELL, 2009, 33 (05) :602-615

[10] Specific Recognition of Linear Ubiquitin Chains by NEMO Is Important for NF-κB Activation [J].

Rahighi, Simin ;

Ikeda, Fumiyo ;

Kawasaki, Masato ;

Akutsu, Masato ;

Suzuki, Nobuhiro ;

Kato, Ryuichi ;

Kensche, Tobias ;

Uejima, Tamami ;

Bloor, Stuart ;

Komander, David ;

Randow, Felix ;

Wakatsuki, Soichi ;

Dikic, Ivan .

CELL, 2009, 136 (06) :1098-1109