Isolation of a Dictyostelium discoideum 14-3-3 homologue

被引：14

作者：

Knetsch, MLW

vanHeusden, GPH

Ennis, HL

Shaw, DR

Epskamp, SJP

SnaarJagalska, BE

机构：

[1] LEIDEN UNIV, INST MOL PLANT SCI, SECT CELL BIOL, NL-2300 RA LEIDEN, NETHERLANDS

[2] LEIDEN UNIV, INST MOL PLANT SCI, SECT YEAST GENET, NL-2300 RA LEIDEN, NETHERLANDS

[3] ROCHE INST MOL BIOL, ROCHE RES CTR, NUTLEY, NJ 07110 USA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH | 1997年 / 1357卷 / 02期

关键词：

cellular slime mold; 14-3-3; developmental regulation; Ftta locus; functional complementation;

D O I：

10.1016/S0167-4889(97)00060-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A 1.0 kb cDNA clone (Dd14-3-3) encoding a 14-3-3 homologue was isolated from a Dictyostelium discoideum cDNA library. The putative Dd14-3-3 protein has highest sequence identity to a barley 14-3-3 isoform (74%). Southern blot analysis suggests that only one 14-3-3 gene is present in the Dictyostelium genome. Highest Dd14-3-3 expression is observed in vegetatively growing cells, and expression decreases during multicellular development. In contrast, Dd14-3-3 protein levels detected immunochemically remained constant during Dictyostelium development. Expression of the Dd14-3-3 cDNA in Saccharomyces cerevisiae complemented the lethal disruption of the two yeast genes encoding 14-3-3 proteins (BMH1 and BMH2). This shows that Dd14-3-3 can fulfil the same function(s) as the yeast 14-3-3 proteins.

引用

页码：243 / 248

页数：6

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