A Gr receptor is required for response to the sugar trehalose in taste neurons of Drosophila

被引:215
作者
Dahanukar, A [1 ]
Foster, K [1 ]
van Naters, WMVDG [1 ]
Carlson, JR [1 ]
机构
[1] Yale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
基金
美国国家卫生研究院;
关键词
D O I
10.1038/nn765
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
We recently identified from the Drosophila genome database a large family of G protein-coupled receptor genes, the Gr genes, and predicted that they encode taste receptors on the basis of their structure and specificity of expression. The expression of Gr genes in gustatory neurons has subsequently been confirmed and 56 family members have been reported. Here we provide functional evidence that one Gr gene, Gr5a, encodes a taste receptor required for response to the sugar trehalose. In two different mutants that carry deletions in Gr5a, electrophysiological and behavioral responses to trehalose were diminished but the response to sucrose was unaffected. Transgenic rescue experiments showed that Gr5a confers response to trehalose. The results correlate a particular taste ligand with a Gr receptor and indicate a role for G protein-mediated signaling in the transduction of sweet taste in Drosophila.
引用
收藏
页码:1182 / 1186
页数:5
相关论文
共 32 条
  • [1] A novel family of mammalian taste receptors
    Adler, E
    Hoon, MA
    Mueller, KL
    Chandrashekar, J
    Ryba, NJP
    Zuker, CS
    [J]. CELL, 2000, 100 (06) : 693 - 702
  • [2] EFFECTS OF CYCLIC-GMP ON THE SUGAR TASTE RECEPTOR CELL OF THE FLY PHORMIA-REGINA
    AMAKAWA, T
    OZAKI, M
    KAWATA, K
    [J]. JOURNAL OF INSECT PHYSIOLOGY, 1990, 36 (04) : 281 - 286
  • [3] T2Rs function as bitter taste receptors
    Chandrashekar, J
    Mueller, KL
    Hoon, MA
    Adler, E
    Feng, LX
    Guo, W
    Zuker, CS
    Ryba, NJP
    [J]. CELL, 2000, 100 (06) : 703 - 711
  • [4] A metabotropic glutamate receptor variant functions as a taste receptor
    Chaudhari, N
    Landin, MA
    Roper, SD
    [J]. NATURE NEUROSCIENCE, 2000, 3 (02) : 113 - 119
  • [5] Candidate taste receptors in Drosophila
    Clyne, PJ
    Warr, CG
    Carlson, JR
    [J]. SCIENCE, 2000, 287 (5459) : 1830 - 1834
  • [6] THE PAIRED BOX GENE POX-NEURO - A DETERMINANT OF POLY-INNERVATED SENSE-ORGANS IN DROSOPHILA
    DAMBLYCHAUDIERE, C
    JAMET, E
    BURRI, M
    BOPP, D
    BASLER, K
    HAFEN, E
    DUMONT, N
    SPIELMANN, P
    GHYSEN, A
    NOLL, M
    [J]. CELL, 1992, 69 (01) : 159 - 172
  • [7] DETHIER VG, 1976, HUNGRY FLY
  • [8] Spatially restricted expression of candidate taste receptors in the Drosophila gustatory system
    Dunipace, L
    Meister, S
    McNealy, C
    Amrein, H
    [J]. CURRENT BIOLOGY, 2001, 11 (11) : 822 - 835
  • [9] GLENDINNING JI, 2000, NEUROBIOLOGY TASTE S, V2, P315
  • [10] PHYSIOLOGY OF A PRIMARY CHEMORECEPTOR UNIT
    HODGSON, ES
    LETTVIN, JY
    ROEDER, KD
    [J]. SCIENCE, 1955, 122 (3166) : 417 - 418