Evaluation of 16S, map1 and pCS20 probes for detection of Cowdria and Ehrlichia species

被引：25

作者：

Allsopp, MTEP ^{[1
]}

Hattingh, CM ^{[1
]}

Vogel, SW ^{[1
]}

Allsopp, BA ^{[1
]}

机构：

[1] Onderstepoort Vet Inst, ZA-0110 Onderstepoort, South Africa

来源：

EPIDEMIOLOGY AND INFECTION | 1999年 / 122卷 / 02期

关键词：

D O I：

10.1017/S0950268899002101

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

A panel of 16S ribosomal RNA gene probes has been developed for the study of the epidemiology of heartwater; five of these detect different cowdria genotypes, one detects five distinct genotypes; one detects any Group III Ehrlichia species other than Cowdria and one detects any Group II Ehrlichia species. These probes have been used on PCR-amplified rickettsial 16S rRNA genes from over 200 Amblyomma hebraeum ticks. Control ticks were laboratory-reared and either uninfected or fed on sheep experimentally infected with different cowdria isolates, field ticks were collected from animals in heartwater-endemic areas. All tick-derived DNA samples were also examined by PCR amplification and probing for two other cowdria genes (map1 and pCS20) which have previously been used for heartwater epidemiology. This paper describes the first direct comparison of all currently available DNA probes for heartwater-associated organisms.

引用

页码：323 / 328

页数：6

共 23 条

[1] Uncharacterized Ehrlichia spp. may contribute to clinical heartwater [J].

Allsopp, BA ;

Allsopp, MT ;

Du Plessis, JH ;

Visser, ES .

VECTOR-BORNE PATHOGENS: INTERNATIONAL TRADE AND TROPICAL ANIMAL DISEASES, 1996, 791 :17-23

[2] Different organisms associated with heartwater as shown by analysis of 16S ribosomal RNA gene sequences [J].

Allsopp, MTEP ;

Visser, ES ;

duPlessis, JL ;

Vogel, SW ;

Allsopp, BA .

VETERINARY PARASITOLOGY, 1997, 71 (04) :283-300

[3] Construction and initial analysis of a representative lambda ZAPII expression library of the intracellular rickettsia Cowdria ruminantium: cloning of map1 and three other Cowdria genes [J].

Brayton, KA ;

Fehrsen, J ;

deVilliers, EP ;

vanKleef, M ;

Allsopp, BA .

VETERINARY PARASITOLOGY, 1997, 72 (02) :185-199

[4]

BRYSON NR, 1997, P UF USAID SADC HEAR

[5]

du Plessis J L, 1971, J S Afr Vet Med Assoc, V42, P217

[6]

DUPLESSIS JL, 1985, ONDERSTEPOORT J VET, V52, P55

[7]

DUPLESSIS JL, 1994, ONDERSTEPOORT J VET, V61, P277

[8]

DUPLESSIS JL, 1987, ONDERSTEPOORT J VET, V54, P319

[9]

DUPLESSIS JL, 1990, ONDERSTEPOORT J VET, V57, P233

[10]

DUPLESSIS JL, 1989, ONDERSTEPOORT J VET, V56, P195

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