Capsaicin-evoked release of pituitary adenylate cyclase activating peptide (PACAP) and calcitonin gene-related peptide (CGRP) from rat spinal cord in vivo

被引:42
作者
Zhang, YZ
Malmberg, AB
Yaksh, TL
Sjolund, B
Sundler, F
Hakanson, R
机构
[1] UNIV CALIF SAN DIEGO, DEPT ANESTHESIOL, LA JOLLA, CA 92093 USA
[2] LUND UNIV, DEPT PHYSIOL & NEUROSCI, SECT NEUROENDOCRINE CELL BIOL, S-22100 LUND, SWEDEN
[3] UNIV LUND HOSP, DEPT REHABIL, S-22185 LUND, SWEDEN
[4] LUND UNIV, DEPT PHARMACOL, S-22100 LUND, SWEDEN
关键词
PACAP; CGRP; peptide; capsaicin; release; rat spinal cord; intrathecal;
D O I
10.1016/S0167-0115(97)02133-2
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Capsaicin-evoked release of pituitary adenylate cyclase activating peptide (PACAP)-like immunoreactivity (LI) from rat spinal cord was examined in vivo. In anaesthetized rats, a catheter was inserted through the atlanto-occipital membrane into the subarachnoid space at the level of the sacral spinal cord for infusion of artificial cerebrospinal fluid. Another catheter was placed in the cisternal opening for outflow. Blood pressure was monitored and kept stable during the experiment. Perfusion samples were analyzed for PACAP and calcitonin gene-related peptide (CGRP) by radioimmunoassay. The addition of capsaicin (10 mu M) to the perfusate elevated the concentrations of PACAP-27-LI in the artificial cerebrospinal fluid by 177%, PACAP-38-LI by 93% and CGRP-LI by 692%. In view of the presence of PACAP-immunoreactive nerve fibres in the superficial layers of the dorsal horn and the expression of PACAP in the small sized neurons in the dorsal root ganglia, the findings suggest that PACAP is released into the artificial cerebrospinal fluid from C-fibres in the spinal card. PACAP conceivably plays a modulating role in nociception. (C) 1997 Elsevier Science B.V.
引用
收藏
页码:83 / 87
页数:5
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