Super-resolution microscopy by nanoscale localization of photo-switchable fluorescent probes

被引：165

作者：

Bates, Mark ^{[2
]}

Huang, Bo ^{[1
,3
]}

Zhuang, Xiaowei ^{[1
,3
,4
]}

机构：

[1] Harvard Univ, Howard Hughes Med Inst, Cambridge, MA 02138 USA

[2] Sch Engn & Appl Sci, Cambridge, MA 02138 USA

[3] Dept Chem & Chem Biol, Cambridge, MA 02138 USA

[4] Harvard Univ, Dept Phys, Cambridge, MA 02138 USA

来源：

CURRENT OPINION IN CHEMICAL BIOLOGY | 2008年 / 12卷 / 05期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/j.cbpa.2008.08.008

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new form of super-resolution fluorescence microscopy has emerged in recent years, based on the high accuracy localization of individual photo-switchable fluorescent labels. Image resolution as high as 20 nm in the lateral dimensions and 50 nm in the axial direction has been attained with this concept, representing an order of magnitude improvement over the diffraction limit. The demonstration of multicolor imaging with molecular specificity, three-dimensional (3D) imaging of cellular structures, and time-resolved imaging of living cells further illustrates the exciting potential of this method for biological imaging at the nanoscopic scale.

引用

收藏

页码：505 / 514

页数：10

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