Broadly tunable dual-wavelength light source for coherent anti-Stokes Raman scattering microscopy

被引：212

作者：

Ganikhanov, F

Carrasco, S

Xie, XS

Katz, M

Seitz, W

Kopf, D

机构：

[1] Harvard Univ, Dept Chem & Chem Biol, Cambridge, MA 02138 USA

[2] Soreq NRC, ElectroOpt Div, IL-81800 Yavne, Israel

[3] High Q Laser Prod GmbH, A-6845 Hohenems, Austria

来源：

OPTICS LETTERS | 2006年 / 31卷 / 09期

关键词：

D O I：

10.1364/OL.31.001292

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

The signal and idler beams from a picosecond, synchronously pumped optical parametric oscillator (OPO) provide the two colors necessary for coherent anti-Stokes Raman scattering (CARS) microscopy. The OPO provides a continuously tunable frequency difference between the two beams over a broad range of Raman shifts (100-3700 cm(-1)) by varying the temperature of a single nonlinear crystal. The near-infrared output (900- 1300 nm) allows for deep penetration into thick samples and reduced nonlinear photodamage. Applications of this light source to in vivo cell and ex vivo tissue imaging are demonstrated. (c) 2006 Optical Society of America

引用

收藏

页码：1292 / 1294

页数：3

相关论文

共 12 条

[1] NONSTATIONARY NONLINEAR OPTICAL EFFECTS AND ULTRASHORT LIGHT PULSE FORMATION [J].

AKHMANOV, SA ;

CHIRKIN, AS ;

DRABOVICH, KN ;

KOVRIGIN, AI ;

KHOKHLOV, RV ;

SUKHORUKOV, AP .

IEEE JOURNAL OF QUANTUM ELECTRONICS, 1968, QE 4 (10) :598-+

[2] PARAMETRIC INTERACTION OF FOCUSED GAUSSIAN LIGHT BEAMS [J].

BOYD, GD ;

KLEINMAN, DA .

JOURNAL OF APPLIED PHYSICS, 1968, 39 (08) :3597-+

[3] Coherent anti-Stokes Raman scattering microscopy: Instrumentation, theory, and applications [J].

Cheng, JX ;

Xie, XS .

JOURNAL OF PHYSICAL CHEMISTRY B, 2004, 108 (03) :827-840

[4] Ordering of water molecules between phospholipid bilayers visualized by coherent anti-Stokes Raman scattering microscopy [J].

Cheng, JX ;

Pautot, S ;

Weitz, DA ;

Xie, XS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (17) :9826-9830

[5] An epi-detected coherent anti-stokes raman scattering (E-CARS) microscope with high spectral resolution and high sensitivity [J].

Cheng, JX ;

Volkmer, A ;

Book, LD ;

Xie, XS .

JOURNAL OF PHYSICAL CHEMISTRY B, 2001, 105 (07) :1277-1280

[6] Temperature-dependent dispersion equations for KTiOPO4 and KTiOAsO4 [J].

Emanueli, S ;

Arie, A .

APPLIED OPTICS, 2003, 42 (33) :6661-6665

[7] Chemical imaging of tissue in vivo with video-rate coherent anti-Stokes Raman scattering microscopy [J].

Evans, CL ;

Potma, EO ;

Puoris'haag, M ;

Côté, D ;

Lin, CP ;

Xie, XS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (46) :16807-16812

[8] Molecular vibration imaging in the fingerprint region by use of coherent anti-Stokes Raman scattering microscopy with a collinear configuration [J].

Hashimoto, M ;

Araki, T ;

Kawata, S .

OPTICS LETTERS, 2000, 25 (24) :1768-1770

[9] Highly nonlinear photodamage in two-photon fluorescence microscopy [J].

Hopt, A ;

Neher, E .

BIOPHYSICAL JOURNAL, 2001, 80 (04) :2029-2036

[10] Simple approach to one-laser, broadband coherent anti-Stokes Raman scattering microscopy [J].

Kee, TW ;

Cicerone, MT .

OPTICS LETTERS, 2004, 29 (23) :2701-2703