In vivo synthesis of meganuclease for generating transgenic zebrafish Danio rerio

被引：6

作者：

Babaryka, A. ^{[1
]}

Kuehn, E. ^{[1
]}

Koester, R. W. ^{[1
]}

机构：

[1] Helmholtz Zentrum Munchen, German Res Ctr Environm Hlth, Inst Dev Genet, D-85764 Neuherberg, Germany

来源：

JOURNAL OF FISH BIOLOGY | 2009年 / 74卷 / 02期

关键词：

Gal4; I-SceI; meganuclease; transgenesis; zebrafish; SCEI MEGANUCLEASE; EMBRYOS;

D O I：

10.1111/j.1095-8649.2008.02075.x

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

The authors show that co-injection at the one-cell stage of mRNA encoding a nuclear-targeted meganuclease I-SceI together with expression cassettes flanked by cognate restriction sites results in efficient stable transgenesis in zebrafish Danio rerio.

引用

页码：452 / 457

页数：6

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