Down-regulation of cellular protein heme oxygenase-1 inhibits proliferation of avian influenza virus H9N2 in chicken oviduct epithelial cells

被引：13

作者：

Qi, Xuefeng ^{[1
]}

Zhang, Huizhu ^{[1
]}

Xue, Tianxia ^{[1
]}

Yang, Bo ^{[1
]}

Deng, Meiyu ^{[1
]}

Wang, Jingyu ^{[1
,2
]}

机构：

[1] Northwest A&F Univ, Coll Vet Med, Yangling 712100, Peoples R China

[2] Shaanxi Prov Engn Technol Res Ctr Vet Biol Prod, Yangling 712100, Peoples R China

来源：

JOURNAL OF GENERAL VIROLOGY | 2018年 / 99卷 / 01期

关键词：

H9N2 avian influenza virus; heme oxygenase-1; chicken oviduct epithelial cells; LAYING HENS; EXPRESSION; REPLICATION; APOPTOSIS; SYSTEM; MODULATION; ACTIVATION; INFECTION;

D O I：

10.1099/jgv.0.000986

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 090105 [作物生产系统与生态工程];

摘要：

The pathogenesis of H9N2 subtype avian influenza virus (AIV) infection in hens is often related to oviduct tissue damage. Our previous study suggested that H9N2 AIV induces cellular apoptosis by activating reactive oxygen species (ROS) accumulation and mitochondria-mediated apoptotic signalling in chicken oviduct epithelial cells (COECs). Heme oxygenase-1 (HO-1) is an inducible enzyme that exerts protective effects against oxidative stress and activated HO-1 was recently shown to have antiviral activity. To study the potential involvement of HO-1 in H9N2 AIV proliferation, the role of its expression in H9N2-infected COECs was further investigated. Our results revealed that H9N2 AIV infection significantly up-regulated the expression of HO-1 and that HO-1 down-regulation by ZnPP, a classical inhibitor of HO-1, could inhibit H9N2 AIV replication in COECs. Similarly, the small interfering RNA (siRNA)-mediated knockdown of HO-1 also markedly decreased the virus production in H9N2-infected COECs. In contrast, adenoviral-mediated over-expression of HO-1 concomitantly promoted H9N2 AIV replication. Taken together, our study demonstrated the involvement of HO-1 in AIV H9N2 proliferation, and these findings suggested that HO-1 is a potential target for inhibition of AIV H9N2 replication.

引用

页码：36 / 43

页数：8

共 25 条

[1]

Phylogenetic analysis of low-pathogenicity avian influenza H6N2 viruses from chicken outbreaks (2001-2005) suggest that they are reassortants of historic ostrich low-pathogenicity avian influenza H9N2 and H6N8 viruses [J].