In addition to SEC11, a newly identified gene, SPC3, is essential for signal peptidase activity in the yeast endoplasmic reticulum

被引：33

作者：

Fang, H

Mullins, C

Green, N

机构：

[1] Dept. of Microbiology and Immunology, School of Medicine, Vanderbilt University, Nashville

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 1997年 / 272卷 / 20期

关键词：

D O I：

10.1074/jbc.272.20.13152

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Among the three characterized subunits comprising the signal peptidase complex of the yeast Saccharomyces cerevisiae (Sec11p, Spc1p, and Spc2p), only Sec11p is essential for cell growth, signal peptide cleavage, and signal peptidase-dependent protein degradation, Here we report the cloning of the SPC3 gene encoding the homolog to mammalian signal peptidase subunit SPC22/23. We find that Spc3p is also required for cell growth and signal peptidase activity within the yeast endoplasmic reticulum.

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页码：13152 / 13158

页数：7

相关论文

共 42 条

[1] PURIFICATION AND CHARACTERIZATION OF HEN OVIDUCT MICROSOMAL SIGNAL PEPTIDASE [J].

BAKER, RK ;

LIVELY, MO .

BIOCHEMISTRY, 1987, 26 (26) :8561-8567

[2] THE YDP PLASMIDS - A UNIFORM SET OF VECTORS BEARING VERSATILE GENE DISRUPTION CASSETTES FOR SACCHAROMYCES-CEREVISIAE [J].

BERBEN, G ;

DUMONT, J ;

GILLIQUET, V ;

BOLLE, PA ;

HILGER, F .

YEAST, 1991, 7 (05) :475-477

[3] ON THE CATALYTIC MECHANISM OF PROKARYOTIC LEADER PEPTIDASE-1 [J].

BLACK, MT ;

MUNN, JGR ;

ALLSOP, AE .

BIOCHEMICAL JOURNAL, 1992, 282 :539-543

[4] TRANSFER OF PROTEINS ACROSS MEMBRANES .1. PRESENCE OF PROTEOLYTICALLY PROCESSED AND UNPROCESSED NASCENT IMMUNOGLOBULIN LIGHT-CHAINS ON MEMBRANE-BOUND RIBOSOMES OF MURINE MYELOMA [J].

BLOBEL, G ;

DOBBERSTEIN, B .

JOURNAL OF CELL BIOLOGY, 1975, 67 (03) :835-851

[5]

BOEKE JD, 1987, METHOD ENZYMOL, V154, P164

[6] SEC11 IS REQUIRED FOR SIGNAL PEPTIDE PROCESSING AND YEAST-CELL GROWTH [J].

BOHNI, PC ;

DESHAIES, RJ ;

SCHEKMAN, RW .

JOURNAL OF CELL BIOLOGY, 1988, 106 (04) :1035-1042

[7] MULTIFUNCTIONAL YEAST HIGH-COPY-NUMBER SHUTTLE VECTORS [J].

CHRISTIANSON, TW ;

SIKORSKI, RS ;

DANTE, M ;

SHERO, JH ;

HIETER, P .

GENE, 1992, 110 (01) :119-122

[8] SIGNAL PEPTIDASES IN PROKARYOTES AND EUKARYOTES - A NEW PROTEASE FAMILY [J].

DALBEY, RE ;

VONHEIJNE, G .

TRENDS IN BIOCHEMICAL SCIENCES, 1992, 17 (11) :474-478

[9] The homologue of mammalian SPC12 is important for efficient signal peptidase activity in Saccharomyces cerevisiae [J].

Fang, H ;

Panzner, S ;

Mullins, C ;

Hartmann, E ;

Green, N .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (28) :16460-16465

[10] C-TERMINAL SEQUENCES CAN INHIBIT THE INSERTION OF MEMBRANE-PROTEINS INTO THE ENDOPLASMIC-RETICULUM OF SACCHAROMYCES-CEREVISIAE [J].

GREEN, N ;

WALTER, P .

MOLECULAR AND CELLULAR BIOLOGY, 1992, 12 (01) :276-282

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