New cationic lipid formulations for gene transfer

被引:66
作者
Liu, F
Yang, JP
Huang, L
Liu, DX
机构
[1] UNIV PITTSBURGH,SCH PHARM,DEPT PHARMACEUT SCI,PITTSBURGH,PA 15261
[2] UNIV PITTSBURGH,SCH MED,DEPT PHARMACOL,PITTSBURGH,PA 15261
关键词
gene transfer; gene therapy; cationic lipid; transfection;
D O I
10.1023/A:1016041326636
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Purpose. To develop appropriate dosage forms of DNA for gene delivery. Methods. 3 beta[N-(N', N' dimethylaminoethane) carbamoyl] cholesterol (DC-Chol) was mixed either with Tween 80 alone, or with additional lipid components including castor oil and phosphatidylcholine (PC) or dioleoylphosphatidylethanolamine (DOPE) to make different lipid formulations. The particle size and the physical stability of the formulations upon mixing with plasmid DNA containing the luciferase cDNA were examined using laser light scattering measurement The transfection activity of the DNA/lipid complexes was tested in presence or absence of serum using a cell culture system. Results. We demonstrated that many favorable properties as a gene carrier could be achieved by formulating DNA into new dosage forms using Tween 80 as the major emulsifier. Compared to the cationic liposomes, these new formulations transfected different cell lines with an equivalent or higher efficiency. Not only are they resistant to serum, but also form stable DNA complexes which could be stored for longer periods of time without losing transfection activity. Conclusions. Cationic lipids formulated into different lipid formulations using Tween 80 as a surfactant appeared to have more favorable physical and biological activities than traditional cationic liposomes as a carrier for gene delivery.
引用
收藏
页码:1856 / 1860
页数:5
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