Immuno-suppressive effect of blocking the CD28 signaling pathway in T-cells by an active component of Echinacea found by a novel pharmaceutical screening method

被引：20

作者：

Dong, GC ^{[1
]}

Chuang, PH ^{[1
]}

Forrest, MD ^{[1
]}

Lin, YC ^{[1
]}

Chen, HM ^{[1
]}

机构：

[1] Acad Sinica, Inst Bioagr Sci, Taipei 115, Taiwan

来源：

JOURNAL OF MEDICINAL CHEMISTRY | 2006年 / 49卷 / 06期

关键词：

D O I：

10.1021/jm0509039

中图分类号：

R914 [药物化学];

学科分类号：

100701 [药物化学];

摘要：

AFTIR (after flowing through immobilized receptor) is a novel method for screening herbal extracts for pharmaceutical properties. Using AFTIR, we identified Cynarin in Echinocea purpurea by its selective binding to chip immobilized CD28, a receptor of T-cells, which is instrumental to immune functioning. The results of surface plasma resonance show that binding between immobilized CD28 and Cynarin is stronger than the binding between CD28 and CD80, a co-stimulated receptor of antigen presenting cells. Cynarin's function was verified by its ability to downregulate CD28-dependent interleukin-2 (IL-2) expression in a T-cell culture line. AFTIR offers promise as an efficient screening method for herbal medicines.

引用

页码：1845 / 1854

页数：10

共 26 条

[1]

THE ANTI-T CELL MONOCLONAL-ANTIBODY 9.3 (ANTI-CD28) PROVIDES A HELPER SIGNAL AND BYPASSES THE NEED FOR ACCESSORY CELLS IN T-CELL ACTIVATION WITH IMMOBILIZED ANTI-CD3 AND MITOGENS [J].